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Effect of Glutathione Adding to Freezing Media on Sperm Parameters and on Malondialdehyde Concentration and Abnormal Sperm Chromatin Percent of Oligozoospermic Patients | ||
| kufa Journal for Nursing sciences | ||
| Article 1, Volume 4, Issue 3, 2014, Pages 1-9 | ||
| Authors | ||
| Hisham Qassim Mohammad Al-Nowainy; Sahib Yahia Almoreshedy; Wafak Gabory Mohammad | ||
| Abstract | ||
| Objective: The study aimed to study the effect antioxidant Glutathione adding to sperm Freezing Media ( Sperm Freeze) on Sperm Parameters and Malondialdehyde concentration and abnormal sperm chromatin percent of Oligozoospermic patients Methodology: This study was performed in the laboratory of Intra cytoplasmic Injection and freezing of sperm in the fertility center of Al-Sader Medical City / Al-najaf Alashraf City during the period from May, 2012 to April 2013 , The study included 30 of Oligozoospermic patients, the statistical analysis of this study performed by using Student's t – test and paired t – test at the level of probability 0.05 Results: The study results shows that used of sperm Freezing Media ( Sperm Freeze) with adding of 1 mm from antioxidant Glutathione leads to a significant difference (p<0.05) in sperm parameters of study included patients , there was a significant increase (p<0.05) in the sperm progressive motility percent and sperm viability percent ,and there was a significant decrease (p<0.05) In The Malondialdehyde concentration and abnormal sperm chromatin percent and no significant difference (p<0.05) In the sperm concentration, normal sperm morphology percent and round cell concentration In comparison with their Means when used of sperm freezing media ( Sperm Freeze) alone . Conclusion: It can be concluded from this study that sperm rapid freezing and thawing processes had a negative effect on the semen and sperm parameters and Malondialdehyde concentration and abnormal sperm chromatin percent in all samples of study included patients and adding of 1 mm from antioxidant Glutathione shows resistance to this negative effect leads to a significant increase (p<0.05) In the studying parameters . Recommendation: using of antioxidant Glutathione with sperm Freezing Media (Sperm Freeze) in sperm cryopreservation processes | ||
| Keywords | ||
| effect; Glutathione; Freezing Media; Sperm Parameters; Malondialdehyde; chromatin; Oligozoospermic | ||
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