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Influence of Caffeine on Human Spermatozoal In Vitro Fertilizing Capacity of Asthenozoospermic Patients | ||
| Al-Mustansiriyah Journal of Science | ||
| Article 1, Volume 21, Issue 5, 2010, Pages 337-343 | ||
| Author | ||
| Essraa M. A. Al Essawe | ||
| Abstract | ||
| The objective of this study was to demonstrate the effect of caffeine on human sperm fertilizing capacity of asthinozoospermic patients. The effect of in vitro caffeine addition to semen of 20 asthenozoospermic infertile patients was studied. .In vitro sperm activation by centrifugation swim up migration technique was used, 3mM caffeine concentration was supplemented to sperm preparation medium prior to sperm activation. Sperm penetration assay (SPA) using zona free hamsters oocytes was performed to determine human sperm fertilizability rate in the presence or absence of caffeine. Twenty normozoospermic semen samples involved as second control .The results showed that the in vitro human sperm penetration rate and penetration index were significantly increased (p<0.001 and p<0.01 respectively) in the caffeine treated group versus control groups. It was concluded that the application of 3mM caffeine to in vitro sperm activation by centrifugation swim up migration technique may be useful for intrauterine insemination and in vitro fertilization, to achieve high pregnancy rate incase of asthinozoospermia | ||
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