Preparation of new HPLC stationary phase and study of their chromatographic performance towered the separation of amino acids and poly aromatic compounds

M. Ali, Noor

	Preparation of new HPLC stationary phase and study of their chromatographic performance towered the separation of amino acids and poly aromatic compounds
Al-Mustansiriyah Journal of Science
Article 1, Volume 21, Issue 6, 2010, Pages 117-128
Author
Noor M. Ali
Abstract
A new stationary phase was prepared by adsorption of Alizarin Red-S solution on Amberlite anion exchanger. The capacity of new prepared resin was 6.65meq./g which is approximately twice as those of Amberlite resin, 3.05meq./g. The resulted polymer has high rigidity with high stability and used as stationary phase for HPLC column. Amino acids (histidine, phenylalanine, tyrosine and tryptophan) were examined with this column with isocratic eluention distilled water adjusted pH at 2.5 and 8.6 as a mobile phase with flow rate of 1.4ml/min and UV detection of 254nm. The retention times for histidine, phenylalanine, tyrosine and tryptophan were 2.18 min, 2.49 min, 2.75min and 3.10 min, respectively at pH 2.5. And when adjusted the pH of mobile phase at 8.6 the retention time. The retention times for these amino acids were 2.45 min, 2.94 min, 3.41min and 3.79 min, respectively. Furthermore some of poly aromatic compounds, such as naphthalene, acenophthene, anthracene, phenatherene and flourine were analyzed with this stationary phase. The eluent 100% methanol and also adjusted pH at 2.5 and 8.6 and with the same condition of flow rate. The retention times for naphthalene, acenophthene, anthracene, phenatherene and flourine were 1.26 min, 1.78 min, 1.54min, 1.9min and 2.82 min, respectively at pH 2.5. and when adjusted the pH of mobile phase at 8.6 the retention time The retention times for polyaromatic compounds were 1.37 min, 1.95 min, 1.74min, 2.29min and 3.79 min, respectively.

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