Study of inhibitory activity for rhamnolipid produced by Pseudomonasaeruginosa against some types of pathogenic bacteria in vitro

Sabah Rezoki Maha Hani Tawfeeq, Nada

	Study of inhibitory activity for rhamnolipid produced by Pseudomonasaeruginosa against some types of pathogenic bacteria in vitro
basrah journal of science
Article 1, Volume 32, Issue 1, 2014, Pages 126-143 PDF (0 K)
Author
Nada Sabah Rezoki Maha Hani Tawfeeq
Abstract
49 isolates for Pseudomonas spp. Bacteria from different sources: pathological (clinically diseased cases) and non-pathological (from contaminated and non-contaminated soil. 21 isolates was diagnosed as P. aeruginosa. Then 2 bacterial isolates was selected (P. aeruginosa PP8 and P. aeruginosa PS14) for rhamnolipid production, first one was from pathological isolates and second was from soil isolates. The rhamnolipid was identified by Thin-layer Chromatography (TLC) and show that the two bacterial isolates produced 3 types of rhamnolipids (mono-, di-rhamnolipid and rhamnolipid A). As well as they diagnosed by High-performance Liquid Chromatography (HPLC). The efficiency of rhamnolipid was tested for bacterial inhibitory activity by measuring the diameter of inhibition zone and its bacterial inhibitory activity was high against Bacillus cersus and P. aeruginosa followed by Staphylococcus aeraus than E. coli respectively. This study also determine the values of minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) for biosurfactants and the results was varied depending on the different type of biosurfactant and type of bacteria with lowest values of MIC and MBC for biosurfactant produced by P. aeruginosa PS42 on the growth of B. cereus bacteria that reached 16 microgram/ml.
Keywords
aeruginosa; bacterial inhibitory activity

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