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Prevalence of VIM Metallo ß –Lactamase among Clinical Isolates of Klebsiella pneumoniae in Hilla Hospitals | ||
| Medical Journal of Babylon | ||
| Article 1, Volume 11, Issue 4, 2014, Pages 825-835 | ||
| Authors | ||
| Eman Mohammad Jarallah; Fatima Moeen Abbas | ||
| Abstract | ||
| This study investigated the presence of blaVIM in clinical isolates of Klebsiella pneumoniae. During the period from April to August 2011, a total of 801 various clinical samples were collected from different hospitals in Hilla city. Of these,117 isolates were diagnosed as K.pneumoniae . High prevalence of K.pneumoniae isolates were detected in stool samples 38 (27%) followed by sputum 19 (15%). All 117 K.pneumoniae isolates were primarily screened for - lactams resistance, 91 (78%) showed positive results for beta lactams . β- lactam resistance isolates were underwent antimicrobial susceptibility to 26 antibiotics by Kirby-Bauer disk diffusion methods .High resistance rate was recorded for penicillins (carbenicillin and ampicillin) (99%) and (94.5%),respectively .Carbapenem resistance was reported in 17 (18.7%) of K. pneumoniae isolates. Phenotypic detection of metallo β- lactamase by imipenem-EDTA disk identified a proportion of 65% as metallo β-lactamase producers. The presence of blaVIM gene was checked by Polymerase Chain reaction (PCR) and confirmed in 14(82.3%) of isolates. | ||
| Keywords | ||
| Klebsiella pneumoniae; VIM metallo; lactamase; Polymerase Chain Reaction | ||
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