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STUDY THE RELATION BETWEEN SELENIUM AND GLUTATHIONE PEROXIDASE ACTIVITY IN IRON DEFICIENT GUINEA PIG. | ||
| Journal of University of Anbar for Pure Science | ||
| Article 15, Volume 7, Issue 3, 2013, Pages 85-89 PDF (414.82 K) | ||
| Document Type: Research Paper | ||
| DOI: 10.37652/juaps.2013.97176 | ||
| Author | ||
| HASAN ALI MUTAR* | ||
| University of Anbar - College of Agriculture | ||
| Abstract | ||
| Little information is available on the relationship of Selenium deposit in target organs and Glutathione peroxidase (GPx) activity in iron deficiency anemia ( IDA). As red blood cells (RBCs) play a crucial role on Se metabolism during Fe deficiency anemia a lower count of RBCs is featured, we aimed to investigate the influence of this pathology on Se bioavailability and the relationship with antioxidant status. 20 male guinea pig were randomly divided into two groups, a control group receiving 100 g diet with normal Fe content (45 mg/kg diet) and the Fe-deficient group receiving 100 g diet with low Fe content (5 mg/kg diet) for 60 days. Both diets were prepared with an adequate Se content (0.180 mg/kg diet). The digestive and metabolic utilization of Se, the distribution in target organ, the GPx activity and thiobarbituric acid-reactive substances (TBARS) production were measured after receiving the diets. Se retention increased (P ≤ 0.001) in the anemic group, fact that contributes to keep the enzymatic antioxidant activity of GPx in normal levels and the tendency observed is that stored Se increased in the organs, especially in kidney (P ≤ 0.01) . The lower count of RBCs featured in this pathology (P ≤ 0.001) causes an increase in kidney deposit is a consequence of the lower urinary losses (P ≤ 0.001 | ||
| References | ||
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[1] Chen C, Yu, et al. The roles of serum selenium and selenoproteins on mercury toxicity in environmental and occupational exposure. Environ Health Perspect 2006; 114:297–301. [2] Renko K, Werner M, Renner-Müller I, Cooper TG, Yeung CH, Hollenbach B, et al. Hepatic selenoprotein P (SePP) expression restores selenium transport and prevents infertility and motor incoordination in Sepp-knockout mice. Biochem J 2008; 409:741–9. [3] Dalir-Naghadeh B, Rezaei SA. Assessment of serum thyroid hormone concentrations in lambs with selenium deficiency myopathy. Am. J Vet Res 2008; 69:659–63. [4] Wintergerst ES, Maggini S, Hornig DH. Contribution of selected vitamins and trace elements to immune function. Ann Nutr Metab 2007; 51:301–23. [5] Goldhaber SB. Trace element risk assessment: essentiality versus toxicity. Regul Toxicol Pharm 2003; 38:232–42. [6] Suzuki KT, Ishiwata K, Ogra Y. Incorporation of selenium into selenoprotein P and extracellular glutathione peroxidase: HPLC–ICP MS data with enriched selenite. Analyst 1999; 124:1749–54. [7] Bügel SH, Sandström B, Larsen EH. Absorption and retention of selenium from shrimps in man. J Trace Elem Med Biol 2001;14:198–204. [8] Kremer D, Ilgen G, Feldmann J. GC–ICP-MS determination of dimethylselenide inhumanbreath after ingestion of 77Se-enriched selenite: monitoring of in-vivo methylation of selenium. Anal Bioanal Chem 2005; 383:509–15. [9] Kuehnelt D, Kienzl N, Juresa D, Francesconi KA. HPLC/vapor generation/ICPMS of selenium metabolites relevant to human urine-selective determination of trimethylselenonium ion. J Anal Atom Spectrom 2006; 21:1264–70. [10] Behne D, Alber D, Kyriakopoulos A. Effects of long term selenium yeast supplementation On selenium status studied in the rat. J Trace Elem Med Biol 2009; 23:258–64. [11] Campos MS, Barrionuevo M, Alférez MJM, Gómez-Ayala AE, Rodríguez-Matas MC, López-Aliaga I, et al. Interactions among iron, calcium, phosphorus and magnesium in nutritionally iron-deficient rats. Exp Physiol 1998; 83:771–81. [12] Reeves PG, Nielsen FH, Fahey GC. AIN-93 purified diets for laboratory rodents: final report of the American Institute of Nutrition and ad hoc Writing Committee on the reformulation of the AIN-76A rodent diet. J Nutr 1993; 123:1939–51. [13] Alferez MJM, Lopez-Aliaga I, Barrionuevo M, Campos MS. Effect of dietary inclusion of goat milk on the bioavailability of zinc and selenium in rats. J Dairy Res 2003; 70:181–7. [14] Hanahan DJ, Ekholm JE. The preparation of red cell ghosts (membranes). Methods Enzymol 1974; 31:168–72. [15] Lowry OH, Rosenburgh NJ, Farr AL, Randall RJ. Protein measurement with folin phenol reagent. J Biol Chem 1951; 193:265–70. [16] Flohé L, Günzler WA. Assays of glutathione peroxidase. Methods Enzymol 1984; 105:114–21. [17] Yagi K. A simple fluorometric assay for lipoperoxide in blood plasma. Biochem Med 1976; 15:212–6. [18] Bjornstedt M, Kumar S, Holmgren A. Selenodiglutathione is a highly efficient oxidant of reduced thioredoxin and a substrate for mammalian thioredoxin reductase. J Biol Chem 1992; 267:8030–4. [19] MacDougall LG. Red cell metabolism in iron deficiency anemia. J Pediatr 1968; 72:303–28. [20] Jotty K, Ojeda ML, Nogales F, Rubio JM,Murillo ML, Carreras O. Selenium tissue distribution changes after ethanol exposure during gestation and lactation: selenite as a therapy. Food Chem Toxicol 2009; 47:2484–9. [21] Ku PK, Ely WT, Groce AW, Ullrey DE. Natural dietary selenium, _-tocopherol and effect on tissue selenium. J Anim Sci 1972; 34:208–11. [22] Suzuki KT, Shiobara Y, Itoh M, Ohmichi M. Selective uptake of selenite by red blood cells. Analyst 1998; 123:63–7. [23] Suzuki KT. Metabolomics of selenium: Se metabolites based on speciation studies. J Health Sci 2005; 51:107–14. [24] Park YC, Kim JB, Heo Y, Park DC, Lee IS, Chung HW, et al. Metabolism of subtoxic level of selenite by double-perfused small intestine in rats. Biol Trace Elem Res 2004; 98:143–57. [25] Behne D, Kyriakopoulos A. Mammalian selenium-containing proteins. Annu Rev Nutr 2001; 21:453–73. [26] Isler M, Delibas N, Guclu M, Gultekin F, Sutcu R, Bahceci M, et al. Superoxide dismutase and glutathione peroxidase in erythrocytes of patients with iron deficiency anemia: effects of different treatment modalities. Coat Med J 2002; 43:16–9. | ||
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