Effect of Ficus carica latex or tumor cell lines in vitro and in vivo in mice

H. Al -  Jeboori, Khalil; A.H. Al . Ghezi, Basim; Y. Yassin, Nahi

	Effect of Ficus carica latex or tumor cell lines in vitro and in vivo in mice
Iraqi Journal of Cancer and Medical Genetics
Article 1, Volume 7, Issue 2, 2014, Pages 187-181
Authors
Khalil H. Al - Jeboori; Basim A.H. Al . Ghezi; Nahi Y. Yassin
Abstract
This study shed the light upon Ficus carica latex from many aspects. The first aspects include the study of the material toxicity on cancer cell lines cultured in vitro which tested on two cancer cell lines which are Hep-2 , AMN-3 with different time of exposure (24 hr. and 48 hr.) and using two fold dilution . The results were read by using neutral red stain and the ratio of absorbance of stain was measured by using ELISA. The result were analyzed by using analysis of variance on level (P<0.05) which were for Hep-2 as follow. The concentration 312.5 up to high concentration show significant difference as compared with control group after 24 hr. of incubation while the concentration 78.2 up to high concentration state significant difference from control group after 48 hr. of incubation. While the line AMN-3 results revealed that the concentration 156.3 up to high concentration show significant difference as compare with control group after 24 hr. while the concentration 78.2 up to high concentration show significant difference from control group after 48 hr. from incubation. The second aspect aimed to study of therapeutic effect of the ficus carica latex on mammary Carcinoma cell line ANM 3 in mice . The mice have successful tumor growth on were classified into (3) groups injected with the same dose which is 5 mg/kg body weigh intra tumor & the 2nd group intra peritoneal & the last lifted as control group . The injection continues for (15) days . The size of tumor was measured along this period . Samples were obtained for histopathological study & the results were analyzed by using analysis of variance where show presence of significant difference between the two treated groups & control group on the level (P < 0.05). The tumor size inhibited ratio was 65.7 % for the intratumor injected group & 59 % for intraperitoneally injected group. The histopathological examination state presence of areas of necrosis, hemorrhage, edema, inflammatory cell infiltration in intratumour injection group and, less extent in intraperitoneally injected group. The study concluded that the Ficus latex had regressive effect on tumor cells lines . Ficus latex had regressive effect on mammary tumor cells inoculated in mice . The regressive ratio of tumor size was more in intratumor injection of Ficus latex than in intraperitoneally injected group .

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