Preparation and Potentiometric Study of Amiloride Hydrochloride Selective Electrodes and Their Application in Determining Some Drugs

Khaleda,; Al-Saidi, H.

	Preparation and Potentiometric Study of Amiloride Hydrochloride Selective Electrodes and Their Application in Determining Some Drugs
Al-Mustansiriyah Journal of Science
Article 1, Volume 21, Issue 6, 2010, Pages 212-229
Authors
; H. Al-Saidi
Abstract
Amiloride hydrochloride ion-selective electrodes were constructed in polymeric membrane by using PVC and based on the use of active ion-pair (Amiloride-phosphotungstate) and the other ion pair (Amiloride- tetraphenyl -borate). The plasticizers used were Di-butyl phthalate (DBPH), Di-octylphthalate (DOP) and Tri-butyl phosphate (TBP). The electrodes (A, B and C) based on the ion pair (Amiloride -phosphotungstic acid) and (D, E and F) based on the ion pair (Amiloride - tetraphenylborate) and the plasticizers used DBPH, DOP and TBP respectively they gave approximately the same linear concentration range from (1x10-5 to 1x10-2) M. The slopes are (54.198, 52.759, 50.910, 49.007, 48.508 and 48.501) mV/decade, and the detection limit were (6×10-7, 1.5×10-6, 7×10-6 , 1.75×10-5, 7×10-6 and 1.5×10-5)M, with the response time (10, 30, 10, 12, 10 and 35) Sec. and the lifetime were about (45, 35, 30, 21, 15 and 10) days respectively. And the working pH range found to be (1.9 –7.8) for the concentration of Amiloride solution 1×10-3 M by using electrode A (Amilo-PT+DBPH). The measurement interferences in the presence of Na+, K+, Mg2+, Mn2+, Cu2+, , Fe3+ and Hydroclorothiazide were studied using separate and mixed methods for selectivity coefficient determination. The pH and life time of the electrodes were also studied. The analytical methods results showed to be simple, rapid and with a good accuracy by comparing it with UV-spectrophotometric method by using F-test.

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