STUDYING THE FREQUENCY OF METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS THROUGH THE MOLECULAR DETECTION OF MECA

Y. Rasheed, Rafeef; S. Abdulamir, Ahmed; H. Raziq, Amir

	STUDYING THE FREQUENCY OF METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS THROUGH THE MOLECULAR DETECTION OF MECA
IRAQI JOURNAL OF MEDICAL SCIENCES
Article 1, Volume 12, Issue 3, 2014, Pages 289-294
Authors
Rafeef Y. Rasheed; Ahmed S. Abdulamir; Amir H. Raziq
Abstract
Background:Methicillin-resistant Staphylococcus aureus is a major cause of serious nosocomial infections and it is very important to have a reliable test to detect these bacteria. mecA encodes the penicillin binding protein 2a, which is associated with staphylococcal methicillin resistance. Objective:The study was to determine the frequency of methicillin-resistant Staphylococcus aureus in different specimens from Iraqi patients and to genetically characterize and type the samples of methicillin- resistant Staphylococcus aureus through the detection of mec A gene. Methods:Sixty clinical isolates of Staphylococcus aureus were submitted to DNA extraction. Genomic DNA was submitted to conventional polymerase chain reaction assays, employing MR1-MR2 primers (primer set). The results were compared to the cefoxitin disks agar diffusion method. Results :Fifty seven of the sixty isolates showed positive results for mecA amplification while three isolates (5%) showed negative results for mecA gene. Conclusion:Good correlation between the mecA gene detection by PCR and the cefoxitin disk diffusion methods was obtained. Key words:mecA gene, Staphylococcus aureus, methicillin resistance Staphylococcus aureus
Keywords
mecA gene; Staphylococcus aureus; methicillin resistance Staphylococcus aureus

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