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EFFECT OF Q-SWITCHED ND: YAG LASER ON THE ACTIVITY AND SPECIFIC ACTIVITY OF Saccharomyces cerevisiae INVERTASE | ||
| IRAQ JOURNAL OF AGRICULTURE | ||
| Article 1, Volume 2, Issue 16, 2011, Pages 0-0 | ||
| Authors | ||
| N. A. Jadah; G. M. Aziz; A. G. Anwer | ||
| Abstract | ||
| This study attempts to estimate the action of Q-switched Nd: YAG laser on activity and specific activity of invertase enzyme produced by Saccharomyces cerevisiae. The yeast cells were irradiated using two wavelengths (532 and 1064) nm of Q-Switched Nd: YAG laser with (1Hz) frequency; Different fluences (11.3, 22.6 and 33.9) mJ/cm2 and different number of pulses (15, 30 and 60 pulses) were used. The irradiated cells were incubated in a liquid nutritive medium for 24 hour. After incubation, the cells were harvested and disrupted to extract the invertase enzyme and its activities were assessed. In comparison with the control, the irradiated cells with fundamental wavelength (1064 nm) showed an increase in the activity and the specific activity of invertase at energy density of 33.9 mJ/cm2 at 15 and 60 pulses, while the irradiation with 532nm at all doses inhibited effect the activity and specific activity of invertase. It is concluded that a fluence at 33.9 mJ/cm2 with 1064 nm wavelength stimulates the activity and specific activity of the enzyme to the maximum values | ||
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