Molecular Detection and Genotyping of Human Papilloma Virus Infections in Iraqi Patients with Esophageal Carcinoma.

Y. Bakir, Tural; MSc,; H. Mohammed Ali, Saad; PhD,; PhD,; M. Jumaa, Intisar; MSc,; W. Ahmad, Dhamraa; B. Hana, Dalya; MSc,; H. Salman, Amal; PhD,; MBchB,; PhD,; H. AL-Khalisi, Maan; MSc,; MBchB,; DDV,; A.  AL-Khafhaji, Fadhil; PhD,; MBchB,; A. Jaumah, Zauhair; MSc,; J. Ahmed, Shayma; PhD,; H. Al-Ubaidi, Gheid; M Turky, Kisma; F. Al-Taei, Wafaa; H. Al-dujaili, Amar; M. Salih, Shahlaa

Y. Bakir, T., MSc, , H. Mohammed Ali, S., PhD, , PhD, , M. Jumaa, I., MSc, , W. Ahmad, D., B. Hana, D., MSc, , H. Salman, A., PhD, , MBchB, , PhD, , H. AL-Khalisi, M., MSc, , MBchB, , DDV, , A. AL-Khafhaji, F., PhD, , MBchB, , A. Jaumah, Z., MSc, , J. Ahmed, S., PhD, , H. Al-Ubaidi, G., M Turky, K., F. Al-Taei, W., H. Al-dujaili, A., M. Salih, S. (2009). Molecular Detection and Genotyping of Human Papilloma Virus Infections in Iraqi Patients with Esophageal Carcinoma.. , 51(2), 204-210.

Tural Y. Bakir; MSc; Saad H. Mohammed Ali; PhD; PhD; Intisar M. Jumaa; MSc; Dhamraa W. Ahmad; Dalya B. Hana; MSc; Amal H. Salman; PhD; MBchB; PhD; Maan H. AL-Khalisi; MSc; MBchB; DDV; Fadhil A. AL-Khafhaji; PhD; MBchB; Zauhair A. Jaumah; MSc; Shayma J. Ahmed; PhD; Gheid H. Al-Ubaidi; Kisma M Turky; Wafaa F. Al-Taei; Amar H. Al-dujaili; Shahlaa M. Salih. "Molecular Detection and Genotyping of Human Papilloma Virus Infections in Iraqi Patients with Esophageal Carcinoma.". , 51, 2, 2009, 204-210.

Y. Bakir, T., MSc, , H. Mohammed Ali, S., PhD, , PhD, , M. Jumaa, I., MSc, , W. Ahmad, D., B. Hana, D., MSc, , H. Salman, A., PhD, , MBchB, , PhD, , H. AL-Khalisi, M., MSc, , MBchB, , DDV, , A. AL-Khafhaji, F., PhD, , MBchB, , A. Jaumah, Z., MSc, , J. Ahmed, S., PhD, , H. Al-Ubaidi, G., M Turky, K., F. Al-Taei, W., H. Al-dujaili, A., M. Salih, S. (2009). 'Molecular Detection and Genotyping of Human Papilloma Virus Infections in Iraqi Patients with Esophageal Carcinoma.', , 51(2), pp. 204-210.

Y. Bakir, T., MSc, , H. Mohammed Ali, S., PhD, , PhD, , M. Jumaa, I., MSc, , W. Ahmad, D., B. Hana, D., MSc, , H. Salman, A., PhD, , MBchB, , PhD, , H. AL-Khalisi, M., MSc, , MBchB, , DDV, , A. AL-Khafhaji, F., PhD, , MBchB, , A. Jaumah, Z., MSc, , J. Ahmed, S., PhD, , H. Al-Ubaidi, G., M Turky, K., F. Al-Taei, W., H. Al-dujaili, A., M. Salih, S. Molecular Detection and Genotyping of Human Papilloma Virus Infections in Iraqi Patients with Esophageal Carcinoma.. , 2009; 51(2): 204-210.

	Molecular Detection and Genotyping of Human Papilloma Virus Infections in Iraqi Patients with Esophageal Carcinoma.
Journal of the Faculty of Medicine
Article 1, Volume 51, Issue 2, 2009, Pages 204-210
Authors
Tural Y. Bakir; ; Saad H. Mohammed Ali; ; ; Intisar M. Jumaa; ; Dhamraa W. Ahmad; Dalya B. Hana; ; Amal H. Salman; ; ; ; Maan H. AL-Khalisi; ; ; ; Fadhil A. AL-Khafhaji; ; ; Zauhair A. Jaumah; ; Shayma J. Ahmed; ; Gheid H. Al-Ubaidi; Kisma M Turky; Wafaa F. Al-Taei; Amar H. Al-dujaili; Shahlaa M. Salih
Abstract
Background: Molecular DNA hybridization has confirmed more than 120 different human papilloma virus (HPV) genotypes. A small group of them have high- risk oncogenic potential. Many studies have described an association of such high risk-HPV genotypes with a variety of esophageal benign tumors as well as malignant squamous cell carcinomas.Patients and Methods: A total number of 90 tissue specimens were collected from 50 patients with esophageal squamous cell (SCC), adenocarcinoma (AC) and carcinoma in situ (CIS); 20 patients with squamous acanthosis (SA); and 20 individuals with apparently-healthy esophageal tissues (AHET). The molecular detection methods for HPV detection and genotyping were performed by in situ hybridization using cocktailed- and specific high- risk HPV DNA probes, respectively.Results: The overall percentage of HPV in the total group of esophageal carcinoma was 20%.The percentage of HPV DNA in the subgroup of SCC and AC was 26.7% and 13.3%, respectively,. However, neither HPV DNA was detected in CIS subgroup nor in both control groups (SA and AHET).The overall genotyping results showed that HPV 18 constituted the majority of the detected high-risk oncogenic HPV genotypes, followed by HPV 16 then HPV 31/33. Keywords Esophageal Carcinoma; Human Papilloma Virus; In Situ Hybridization
Keywords
Esophageal Carcinoma; Human Papilloma Virus; In Situ Hybridization; Fas and Fas; Ligand in trophoblasts; diabetes; pancreatic regeneration; pancreatic stem cells; replication; Alpha; feto protein; Blood glucose; DNA; Eukaryotes; extraction; mice; bone marrow; sperm; Hind III digestion; tumor marker; stomach cancer; colon cancer; rectum cancer; Infectious mononucleosis; Marker profile and EBV

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