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USING A DAUL BACTERIAL INOCULANT As BIO-FERTILIZER FOR IRAQI WHEAT | ||
| IRAQ JOURNAL OF AGRICULTURE | ||
| Article 1, Volume 16, Issue 6, 2011, Pages 0-0 | ||
| Authors | ||
| K.H. Motlag; M.T. Al-Kaisey; H.M. Al-Obaidy | ||
| Abstract | ||
| Twelve isolates of Rhizobium spp. were isolated from root nodules of four legumes plants (broad bean, mung bean, alfalfa and clover) from the middle of Iraq. Three different combinations of this isolates with the recommended bacterium (A. brasilense) were selected based on their efficiency in nitrogen fixation (root nodules number/plant) using tube culture technique and pots experiment with 50% chemical fertilization as manifested by plant growth parameters (dry weight, productivity and the percentage of protein in the seeds), compared to 100% chemical fertilization as control treatment. The results showed that twelve isolates of Rhizobium spp. were isolated, four isolates from each of R. leguminosarum (broad bean and mung bean), R. meliloti (alfalfa) and R. trifolii (clover). Nitrogen fixation test (number of nodules/plant) showed that isolate RL2 from the bacterium R. leguminosarum isolate RM1 from the bacterium R. meliloti and isolate RT3 from R. trifolii were the superior recorded 16.3, 29.0 and 24.0 nodule/plant, respectively. The dual inoculant of these superior isolates and recommended isolate of wheat A. brasilense (1:1) under 50% chemical fertilization, revealed that Az. +RL2 was the best one which induced significant increment in growth parameters dry weight 8.59 gm/pot seed protein percentage 12.83%, seed productivity 19.43 gm/pot compared to control (100% chemical fertilization) which were 6.93 gm/pot and protein 12.3%, respectively. ……………… | ||
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