In this research five local isolates were used from the fungus B .bassiana collected from different regions from Iraq for test the pathogenecity of them on different stages of corn stem borer (eggs, first larval stages and fifth larval stages). The pathogenisity test of B. bassiana isolates indicated that all isolates were efficient in the infection degree of different stages of corn stem borer. The percentages of the first and fifth larval stages ranged between 94.12-100%
The effect of various temperatures degrees on the growth rate of the fungal isolates was also studied, indicated that the best growth temperature of local isolates was 30ºc.The mean growth diameter was 71.3, 55.5, 68.2, 64.6 and 66.58 mm after 20 day at 30ºc for the isolates BSA1, BSA2, BSA3, BSA4 and BSA5 respectively. The results also showed that the mean growth rate of all isolates were decreased at 20ºc and 25ºc while the lowest means growth were observed at 10 ºc which was 9.3-10mm after 20 days from treatment. The growth of all the isolates was stopped at 35ºc temperature which influenced the growth rate. The growth rate was 0.5-0.49, 1.39-1.57, 2.08-3.08 and 2.78-3.57 mm/day at the temperature 10, 20, 25, and 30ºc respectively. The highest average spores production for the various isolates was at 30ºc which reached 30.5, 26.25, 23.69, 23.75 and 33.5×107 spore/cm2 for the isolate BSA1, BSA2, BSA3, BSA4 and BSA5 respectively while (1.29, 1.19, 1.59, 1.02 and 1.25) and (1.72, 1.45, 1.29, 1.58 and 1.48×107) spore/ cm2 at 20, 25 ºc respectively. All isolates gave no spores at 10 and 35ºc. |