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Effect of electric current and ultra violet of elements and protein in shoot of three Hybrids of Cucumis sativus L. In vitro | ||
| Jornal of Biotechnology Research Center | ||
| Article 1, Volume 9, Issue 1, 2015, Pages 15-23 | ||
| Authors | ||
| Abedaljasim M; ; Al- Saadi Abas A; Al-Bassam Suha M | ||
| Abstract | ||
| Three hybrids of American cucumber were studied, they are Lot number EM285 labeled number 1, Hybrid tocaeen F1 labeled number 2, Hybrid super green F1 labeled number 3. The seeds of these hybrids were exposure to four frequencies of electric current 0,1,2 and 2.5 Am for 5 minutes and 4 periods of ultra violate rays UV at 254 nm. For 0,30,60 and 90 second. The sterilized seeds were cultured in MS medium. After 3 weeks apical meristems of seedling were excise and cultured in new MS medium and of 5 weeks shoot systems were taken to study the element content nitrogen, phosphorous, potassium, calcium and magnesium in addition to estimate protein percentage. The results showed that hybrid 3 had a significant increase of element contents and protein percentage compared with hybrid1 and 2.The interactions between hybrid3 and 90 sec. UV exposure showed the highest content of calcium element reached 2.73 mg.gm-1 with an increase of 184.37%compared with the control. The interaction between 2.5 Am and 90 sec. UV exposure showed the highest rate of protein reached 15.5%with significant differences of all interactions. | ||
| Keywords | ||
| Electric current; ultra violate rays; cucumber | ||
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