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Effect of Naphthalene acetic acid and date palm pollen extraction on gene expression on fruit of Hillawi date palm cultivar during growth and ripening | ||
| Jornal of Biotechnology Research Center | ||
| Article 1, Volume 9, Issue 1, 2015, Pages 37-43 | ||
| Author | ||
| Aqeel Hadi Abdulwahed | ||
| Abstract | ||
| This study was carried out on fruit of Hillawi date palm cultivar which pollinated by Khukri Adi and Ghunami Akhder cultivars to study the effect of NAA 25 ppm and pollen extraction of Khukri Adi and Ghunami Akhder 2g/L which were sprayed at 30 and 45 day after pollination on gene expression by studying the changes in protein pattern during fruit development and ripening by gel electrophoreses method with sodium dodecyle sulfate (SDS-PAGE). The result showed there were many protein band which were different in molecular weight and numbers on the gel with changes on fruit growth stages and treatments, These bands of protein were similar in early stage of growth but different in the period of 13,15 weeks after pollination, this difference may be correlated with ripening date. Our results showed that we could consider the protein pattern as gene expression markers for fruit and linking them with a different growth and ripening stage and explanation of metaxenia phenomenon. | ||
| Keywords | ||
| date palm; Pollen; Naphthalene acetic acid; fruit | ||
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