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Efficient Regeneration of Carrot (Daucuscarota L.) plants from cell suspension derived-callus | ||
| Jornal of Biotechnology Research Center | ||
| Article 1, Volume 9, Issue 1, 2015, Pages 58-63 | ||
| Authors | ||
| Amjad Abdul-Hadi Mohammed; MozahimKassim Al-Mallah | ||
| Abstract | ||
| This study succeeded in establishment carrot cell suspension cultures from stem callus in liquid MS medium containing 1.0 mg L-1 of each NAA and BA. Its density approach 3.4 ×105 cell ml-1 at the third day of culture. These cells,continued, when embedded in agar drop using Multiple Drop Array (MDA) technique, in division and forming cellular colonies which producing numerous callus primordia that developed to callus cultures. Whentransferred to the solid differentiation medium (MS+ 1.0 mg L-1 NAA+ 1.5 mg L 1 BA),two hundreds and seventy-three shoots produced. They readily rooted in agar-solidified MSO medium after three weeks and adapted in conditions of culture room. They were nottransferred to field due to the unfavorable environmental conditions at that time. Cell suspension-derived callus tissues contained 82.62 μg anthocyanin gm-1 of callus fresh weight, and 0.555 mg beta-carotene 100 gm-1 of callus fresh weight in the third period of extraction compared with their quantitycalculated in stems callus which recorded 52.4 μg gm-1 and 1.988 mg gm-1 respectively. | ||
| Keywords | ||
| carrot plants; Multiple drop array; cell suspension callus; beta; carotene; anthocyanin | ||
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