Hafedh Abbas, A., LikaaHasounSagban, , Hussein Obaid, H., Abdul Ameer, I., Hussein Kubba, R., Adnan Ez-Aldeen, S. (2015). The mutagenicityand anti-mutagenicity effect of aqueousleaves extract of Vincarosea by bacterial system (Part one). , 9(1), 21-29.
Ali Hafedh Abbas; LikaaHasounSagban; Hind Hussein Obaid; Ishrak Abdul Ameer; Rasha Hussein Kubba; Shaymaa Adnan Ez-Aldeen. "The mutagenicityand anti-mutagenicity effect of aqueousleaves extract of Vincarosea by bacterial system (Part one)". , 9, 1, 2015, 21-29.
Hafedh Abbas, A., LikaaHasounSagban, , Hussein Obaid, H., Abdul Ameer, I., Hussein Kubba, R., Adnan Ez-Aldeen, S. (2015). 'The mutagenicityand anti-mutagenicity effect of aqueousleaves extract of Vincarosea by bacterial system (Part one)', , 9(1), pp. 21-29.
Hafedh Abbas, A., LikaaHasounSagban, , Hussein Obaid, H., Abdul Ameer, I., Hussein Kubba, R., Adnan Ez-Aldeen, S. The mutagenicityand anti-mutagenicity effect of aqueousleaves extract of Vincarosea by bacterial system (Part one). , 2015; 9(1): 21-29.

The mutagenicityand anti-mutagenicity effect of aqueousleaves extract of Vincarosea by bacterial system (Part one)

Jornal of Biotechnology Research Center
Article 1, Volume 9, Issue 1, 2015, Pages 21-29
Authors
Ali Hafedh Abbas; ; Hind Hussein Obaid; Ishrak Abdul Ameer; Rasha Hussein Kubba; Shaymaa Adnan Ez-Aldeen
Abstract
This study was carried out in order to determine the toxic, mutagenic and anti-mutageniceffects for aqueous extract of Vincaroseadried leaves against the mutagenic effect of Methotrexate (MTX) as a chemical mutagen and Ultra Violet rays (UV) as a physical mutagen. The effect was studied in a bacterial system (G-system). The used system consisted of three wild isolates G3Bacillus spp., G12Arthrobacter spp.and G27Brevibacterium spp., The studydepended on recording survival fraction (Sx) as an indicator for the induction of Streptomycin and Rifampicin resistance mutants as a genetic marker.Aqueous extract was prepared from dried leaves, gradual concentrations of plant aqueous extract was used to choose the suitable concentration which is remembered the negative control, the optimum concentration of aqueous extract was 200 µl/ml comparing with the negative control. The interactions included three types of treatments (pre-mutagen, with-mutagen and post-mutagen) in order to determine the mechanisms of this plant extracts in preventing or reducing the genotoxic effect of mutagen (MTX and UV).The results showed that the interaction effect between the optimum concentration of aqueous extract and the mutagen on survival fraction (Sx) increase the survival fraction value of the of G-system isolates to reach normal value compare with positive control (MTX), the results of the interaction between optimum concentration for extracts and the treatment with mutagen to induce resistance mutant for streptomycin and rifampicin found that the MTX had no effect to induce resistance mutant for these two antibiotics, for all types of treatment (pre-MTX, with-MTX, and post-MTX), the aqueous extract suppress or repair mutant and give 100% protection for bacterial cells, while the UV had some effects to induce resistance mutant for these two antibiotics for pre-UV and within-UV treatments and the aqueous extract showed suppressing or repairing mutant partially with post-UV treatment and gave 100% protection for bacterial cells.
Keywords
Catharanthus roseus; Vinca rosea; Aqueous extract; Anti; Mutagenicity
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