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RT PCR Detection and Propagation of Respiratory Syncytial Virus in Human Lung Carcinoma Cells (A549) cell line | ||
| Iraqi Journal of Science | ||
| Article 1, Volume 56, Issue 1, 2015, Pages 69-74 | ||
| Authors | ||
| Layla F. Ali; Raghad G. Al-Suhail; Faisal G. Naser | ||
| Abstract | ||
| In this study negative result of real-time reverse transcription-QPCR (RT-PCR) assay tests of Influenza virus of nasal screetion and throat swap samples of Iraqi patients hospitalized with signs and symptoms of an upper respiratory tract infection in Central Republic Health Laboratory in Iraq were tested for Respiratory Syncytial Virus infection by RT PCR .Positive samples was 4 out 0f 20 were used .Viral isolation was done on a monolayer of 70-80% confluent Human Lung Carcinoma Cells (A549) cell line and incubated at 33ºC for 4 days .Syncytia was observed in 3 positive samples | ||
| Keywords | ||
| RT PCR; RSV; Tissue culture | ||
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