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STUDY THE ABILITY of Pasteurell multocida ANTIGEN TO STIMULATE THE IMMUNO RESPONSE OF BLOOD LYMPHOCYTES CELLS AND QUITE THE CELL CYCLE PROGRESSION | ||
| IRAQ JOURNAL OF AGRICULTURE | ||
| Article 1, Volume 14, Issue 1, 2009, Pages 0-0 | ||
| Authors | ||
| S. S. Kadhum; E.K. Shubber; D. S . Saleh | ||
| Abstract | ||
| This study was carried out to characterize the immunity of whole cell killed antigen of P. multocida by using blood lymphocyte transformation assay through DNA-Labeling with bromodeoxy uridine for measuring cell division and sister chromatide exchange (SCE) formation. Different concentrations of the crud antigen were used to stimulate cells isolated from human, sheep and goats. The results show that there is a significant variation in blood lymphocyte blastegnesis as a function of concentration of antigens. There was increasing in blood lymphocyte blastegnesis BI%, mitotic activities MI% (replication activityRI %) with decrease of antigens concentration. The blood lymphocytes from goats were expressed as a highest response to antigen than that cells from human and sheep, however these antigens have not effective on the spontaneous level of sister chromatide exchange in either cell types. These results indicate that blood cell transformation method "Brdurd" more practical, accurate and effective method for evaluation of antigenesity. | ||
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