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EFFECT OF SODIUM AZIDE ON THE ABILITY OF Glycine Max L. CALLUS TO SALT TOLERANCE In Vitro | ||
| IRAQ JOURNAL OF AGRICULTURE | ||
| Article 1, Volume 2014, Issue 2, 2009, Pages 0-0 | ||
| Authors | ||
| K. M. Ibrahim; H. K. M. Al; ; S. H. Mahmod | ||
| Abstract | ||
| Cotyledons and embryos excised from mature seeds of two soybean (Glycine max L.) varieties Taka 2 and Lee 74 were cultured on modified (MS) medium supplemented with different growth regulators for callus initiation. Callus was then exposed to different concentrations of salt mixtures, namely NaCl, CaCl2 and MgCl2 in a ratio of 2:2:1 at concentrations of 0, 50, 100, 150, 200 or 250 mM added to the culture medium. Callus cultures were exposed to the chemical mutagene Sodium Azide (SA) at concentrations 0, 0.05, 0.5 or 1 mM for 30, 60, 90 or 120 min. Results showed no significant differences between the two varieties in their response to SA. The treatment with SA led to an increase in fresh and dry weight of the callus at (0.05 and 0.5 mM) compared with untreated one. Treatment with 1 mM was considered as a lethal and 0.5 mM as a sub lethal, therefore the later was adopted to generate variation for a period of 90 minutes. Exposure of callus for 120 minutes resulted in a very friable callus. Callus cultures treated with 0.5 mM of SA for 90 minutes were cultured on modified MS medium containing (0 – 250) mM salt mixtures. Results showed that salt tolerance in callus cultures increased as expressed in callus fresh weight compared with the untreated callus. In a separate experiment, callus cultures were treated with (0 or 0.5) mM of SA for 90 minutes, then grown on MS medium supplemented with (200) mM of salt mixture. Results revealed that fresh and dry weight of calli were higher in SA treated cultures only. SA treated callus combined with salinity treatment was significantly higher than those treated with salinity only. | ||
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