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Staphylococcal nuclease removes Escherichia coli and Klebsiella pneumoniae previously adhered to uroepithelial cells | ||
| Iraqi Journal of Science | ||
| Article 1, Volume 56, Issue 1, 2015, Pages 367-378 | ||
| Authors | ||
| Hind T. Ibraheem; Harith J. F. Al-Mathkhury | ||
| Abstract | ||
| Twenty-seven S. aureus isolates were obtained from patients referring various hospitals in Baghdad. Only 17 isolates produced DNase. SNase was extracted and purified from Saphylococcus aureus 3 isolate since it produces the largest zone of clearance on DNase agar. Nevertheless, only those phenotypically-producer of DNase harboured nuc gene. Present study revealed that the crude enzyme had a specific activity of 50.66 unit/mg; while it reached 241 unit/mg after ion exchange chromatography using carboxymethyl cellulose column. SDS-PAGE showed a single sharp band with an approximately 16.8 kDa molecular weight. A matter indicates that the enzyme is consistently pure. Results proved that SNase was able to significantly (P< 0.05) reduce the number of the uropathogens; Escherichia coli and Klebsiella pneumoniae attached to the uroepithelial cells. | ||
| Keywords | ||
| Staphylococcus nuclease; coli; pneumoniae; uroepithelial | ||
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