EXTRACTION AND PURIFICATION OF UREASE IN THE SEEDS OF WHEAT Triticium aestivum L.

F. A. Al-Jumaily, E.; O. F. Al-Karky, L.; F. Hassan, B.

	EXTRACTION AND PURIFICATION OF UREASE IN THE SEEDS OF WHEAT Triticium aestivum L.
IRAQ JOURNAL OF AGRICULTURE
Article 1, Volume 14, Issue 4, 2009, Pages 0-0
Authors
E. F. A. Al-Jumaily; L. O. F. Al-Karky; B. F. Hassan
Abstract
The optimum extraction conditions were determined to extract Urease from Wheat seeds Triticum aestivumL. (Local variety cultivar) using different solvent and buffers with different concentrations and pHs values. It was found that phosphate buffer (20 mM) at pH 7.5 with 1m M EDTA; 1m M β-mercaptoethanol; 1m M PMSF under cooling conditions (4oC) and 4 hours extraction time yielded an enzyme extract with specific activity of 961.57 unit/mg protein; while using the same buffer solution under cooling conditions (4oC) and for 24 hours extraction resulted a value of 843.09 unit/mg protein. Urease extracted from Wheat seeds was purified by the following steps:precipitation by ammonium sulphite (40-60)% Saturation and dialyzed against phosphate buffer 20 m M at pH 7.5. The obtained extract was introduced DEAE–Cellulose column followed by gel Filtration through Sephacryl S-200 column. The enzyme purification fold and yield were 41.93 and 36.67% respectively.

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