Effect of prolactin (PRL) gene polymorphism for Dams in growth traits of Turkish Awassi lambs

H. S. Al-Ithawi, R.

	Effect of prolactin (PRL) gene polymorphism for Dams in growth traits of Turkish Awassi lambs
Al-Anbar Journal of Veterinary Sciences
Article 1, Volume 7, Issue 1, 2014, Pages 20-25 PDF (694.1 K)
Author
R. H. S. Al-Ithawi
Abstract
This study was conducted to investigate the effect of prolactin gene polymorphism in Turkish Awassi ewes on body weight and some body measurements of their lambs. The DNA of45 ewes were extracted and the body weights and measures were recorded in birth, weaning and 6 month's ages. Primers were supplied for amplifying the specific segment (INTRON2). Polymerase Chain Reaction (PCR) was done after finding the best condition to perform the reaction. The specific segment amplified well. RFLP markers were used for detecting the polymorphisms of the segment. Its analysis was performed by incubating of PCR product by HaeIII restriction enzyme at 37°C for 4 h. Gels were visualized using 1.5% agarose, and the distribution percentage of polymorphism of the intron2 in PRL gene of ewe's sample were 13.3, 51.11 and 35.56% for AA, AB and BB genotype respectively. The variation among these percentages was highly significant (P≤0.01). The effect of this polymorphism was significant (P≤0.05) on lambs birth, weaning and 6months age weight, body length and height at front were significant (P≤0.05) or highly significant (P≤0.01), but the remaining measurements were non significant. In conclusion gene polymorphism of PRL gene revealed possibility of adopted in sheep improvement strategy to increase the economic gain in the breeding schemes.
Keywords
Turkish Awassi lambs; gene polymorphism; prolactin; growth traits; RFLP

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