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Molecular Detection of Serotypes K1 and K2 of Klebsiella pneumonia Isolated Form Wound and Burn Infections | ||
| Al-Anbar Journal of Veterinary Sciences | ||
| Article 1, Volume 9, Issue 1, 2016, Pages 1-7 PDF (799.33 K) | ||
| Authors | ||
| O. N. Flaih; L. M. Najeb; R. K. Mohammad | ||
| Abstract | ||
| This study has included the molecular detection of K. pneumonia between serotype K1 and K2. A total of (34) sample were collected from different clinical specimens wounds and burn during the period from December 2014 to April 2015 isolated was general alramadi hospital education. 7 isolated was of the most multi drug resistance to antibiotic. seven isolates of K. pneumonia were identified by using PCR technique for amplification of magA a specific primer for serotype(K1), and rcsA a primer for capsule genes (K2). The results showed that five isolates (71.4%) of K. pneumonia belong to the K2 serotype due to a clear band with a molecular size 254bp for rcsA. whereas neither magA had not given a band, therefore no isolate was belonging to K1 serotype. Thus the rest isolates (28.6%) of K.pneumonia were neither K1 nor K2 and identified as serotype Non-K1/K2 | ||
| Keywords | ||
| Klebsiella pneumonia; Serotypes K; PCR | ||
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