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Effect of adding carnitine and inositol to Tris extender on post-cryopreservative semen quality of Holstein bulls | ||
| Al-Anbar Journal of Veterinary Sciences | ||
| Article 1, Volume 9, Issue 1, 2016, Pages 8-18 PDF (915.99 K) | ||
| Authors | ||
| T. A. Abdulkareem; O. A. Mohamed; A. M. H. Shubber; F. F. Ibrahim; W.Y. Lataf | ||
| Abstract | ||
| Abstract This study was undertaken at the Department of Artificial Insemination, Abu-Ghraib belong to the Directorate of Animal Resource, Ministry of Agriculture, during the period from October 2012 to February 2013 to investigate the effect of addingcarnitine and inositol to Tris extender on post-cryopreservative semen characteristics forHolstein bulls following different periods. Seven mature bulls of 2.5-3 years old were used in this study. Semen was collected via artificial vagina in one ejaculate per bull per week for the 7 weeks experimental period. The evaluations were performed for fresh semen, and thereafter, pooled, and subdivided to different experimental groups by diluting with Tris-based extender. Carnitine (7.5 mM), inositol (7.5 mM) were added to Tris extender in the second and third groups, while the Tris was added only to the control group.Sperm cell individual motility, live sperm cells percentage, total sperm abnormalities percentage along with the acrosome integrity percentage were investigated following different periods (cooling at 5 °C, 48 hrs, 1, 2 and 3 months post cryopreservation, PC). Adding of carnitine and inositol had significantly(P≤ 0.05) increased the sperm cell individual motility, three months PC as compared with the control group. Carnitine had increased (P≤0.05) live sperm cells percentage, three months PC in comparison with both control and Inositol groups. On the other hand, adding of carnitine and inositol decreased (P<0.05) total sperm cells abnormality percentage as compared with control group throughout the all PC experimental periods. Higher (P≤ 0.05) acrosome integrity percentage was resulted by adding carnitine and inositolduring all preservation periods as compared with the control group. In conclusion, adding of carnitine and inositol to Tris extenderimproved the PC semen characteristics of Holstein bulls through their pivotal role as antioxidant and cryoprotectant agents. These will certainly enhance the conception and pregnancy rates of inseminated cows | ||
| Keywords | ||
| Carnitine; Inositol; Semen; Cryopreservation; Bulls | ||
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