CELLULASES PRODUCTION FROM LOCAL ASPERGILLUS SP. ISOLATE AND ASSESING SOME OF THEIR PROPERTIES AND APPLICATION  2- STUDYING OPTIMUM CONDITIONS FOR CELLULASES PRODUCTION FROM ASPERGILLUS SP. AI8

H.A, Aswan; Th. Alrawi, Akram

	CELLULASES PRODUCTION FROM LOCAL ASPERGILLUS SP. ISOLATE AND ASSESING SOME OF THEIR PROPERTIES AND APPLICATION 2- STUDYING OPTIMUM CONDITIONS FOR CELLULASES PRODUCTION FROM ASPERGILLUS SP. AI8
The Iraqi Journal of Agricultural Science
Article 1, Volume 38, Issue 3, 2007, Pages 24-35
Authors
Aswan H.A; Akram Th. Alrawi
Abstract
A series of experiments were conducted to explore the optimal conditions for cellulases production including carbon sources, nitrogen sources, initial inoculum size, initial pH and temperature. Maize car cobs, wheat straw, rice hulls, date peels, filter paper, cotton, molasses, date syrup and cellulose were used as carbon sources, some of them were treated with alkaline peroxide solution. Analyzed heated yeast, ammonium sulphate, peptone, sodium nitrate, ammonium chloride, yeast extract and corn steap liquor were used as nitrogen sources. Different inoculum sizes were used (102,103,10 lOS, 106, 107) spore/gm., also different pH were used (3,5,5.5,6,6.8,7,8) and different degrees of temp. Were used (25, 30, 35, 40, 45, 50)°C. It was found that the highest activity was achieved by using a medium containing: 1 % of treated wheat straw by alkaline peroxide, in addition to, 0.1 % molasses, 0.3% NH4C1, 0.05% MgS04.5H20, 0.05% KCI, 0.01% FeS04.7HzO and 0.1 % K2HP04 with initial pH of 8.0. The preferred size of inoculum was 1 xl05 spore/gm with a fermentation temp of 30°C. In conclusion, we can use natural sources and industrial wastes as carbon sources for cellulases production to get the target to save the environment from pollution.

Statistics Article View: 163 PDF Download: 28