The synergistic Influence of some antioxidants added to Tris extender on sperm cells individual motility of Holstein bulls following different cooling and cryopreservation periods

A. Abdulkareem, T.; S. Noon, M.; H. Sultan, K.

	The synergistic Influence of some antioxidants added to Tris extender on sperm cells individual motility of Holstein bulls following different cooling and cryopreservation periods
Al-Anbar Journal of Veterinary Sciences
Article 1, Volume 10, Issue 1, 2017, Pages 10-20 PDF (953.38 K)
Authors
T. A. Abdulkareem; M. S. Noon; K. H. Sultan
Abstract
This study was conducted to explore the synergistic effect of adding some antioxidants to Tris extender on sperm cells individual motility of Holstein bulls following different cooling and cryopreservation periods (5°C, 48 h, 1 and 2 months post cryopreservation, PC). This experiment was executed at the Artificial Insemination Department; belong to the Animal Resources Directorate, Ministry of Agriculture in Abu-Ghraib region (25 Km west of Baghdad) during the period from 15th November 2015 to 30th March 2016. Semen was collected via artificial vagina in one ejaculate per bull per week for the 6-week experimental period. Pooled semen was equally divided into five groups. Was added to the first group (A1) diluted Tris just where I went back the control group, Combinations of vitamin C + carnitine (5 and 7.5 mm/ ml, respectively; A2), vitamin E + coenzyme Q10 (0.2 and 0.5 mm/ ml, respectively; A3), vitamin E + α-lipoic acid (0.2 and 0.5 mm/ ml, respectively; A4) and carnitine + catalase (7.5 mm and 100 IU / ml, respectively; A5). The individual motility (IM) was greater (P≤ 0.01) in the A5 as compared with the remaining groups at 1 and 2 months PC (44.16 ± 3.00 %). Moreover, the IM was also greater (P≤ 0.01) in A5 as compared with the A1, A2 and A4 groups at the 48 h PC time period. However, the IM was not significantly different in all groups at the cooling time period. As the percentage of individual movement of sperm was significantly different between the extended conservation at A5 Group did not differ. The Group A2 has recorded the lowest individual movement of the sperm after 48 hours of conservation freeze-reaching 22.50 ± 3.81%. In conclusion, the synergistic effect of adding some antioxidant combinations had improved sperm cells individual motility of Holstein bulls, which will positively reflect in enhancing bull’s fertility and owner’s economic returns consequently
Keywords
Antioxidants; semen freezability; individual motility Holstein bulls

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