- All Journals
- Al-Qadisiyah University
- Basrah University
- Kirkuk University
- Mosul University
- The Iraqi Borad for Medical Specialization
- University of Anbar
- University of Technology
- Al Mustafa University
- Al-Zahraa University for Women
- Alnoor University
- Kunooz University
- Misan University
- Ninevah University
- Tikrit University
- University of Al-Hamdaniya
- University of Kerbala
Relationship of Lectins Pseudomonas aeruginosa bacteria with some other virulence factors | ||
| Al-Anbar Journal of Veterinary Sciences | ||
| Article 1, Volume 10, Issue 1, 2017, Pages 108-118 PDF (843.9 K) | ||
| Authors | ||
| A. N. Hamid; S. A. L. Al Maeny; M. I. Nader | ||
| Abstract | ||
| One hundred fifty-two 152 clinical samples were collected from some Baghdad hospitals. About cystic fibrosis samples, 6 samples were obtained from post graduate students in Institute of Genetic Engineering. From different sources, 62 isolates of Pseudomonas aeruginosa had been identified. The results of electrophoresis for PCR productions of gene 16S rRNA, which considered identification gene, showed peaks in the suspected size (956 bp) in all isolates. Fifty-four 44 isolates, were selected to investigate the Susceptibility Test toward 11 types of antibiotics and the bacterial isolates exhibited a high resistance against Ampicillin, Amoxicillin and Nalidixic acid; then decreased against Cefotaxime, Ceftazidimim, Tetracycline, Amikacin, Augmantin, Vancomycin, and Chloramphenicol, while the antibiotic Imipenem recorded a low resistance. Also, 17 isolates were selected for testing their capability to produce Lectins by the agglutination with human RBCs in their groups (A, B, and O), and testing their capability for production of protease, hemolysin, and pyocyanin. Indeed, Lectins extracts of P. aeruginosa strain showed variance agglutination activity toward human RBCs at standard ranged from zero to 128. The results revealed finding variance capabilities in activity of protease of the bacteria isolate P. aeruginosa ranging from 0-340 unit/ml. Those isolates exhibited a variance in the hemolysin activity at standard ranged from 0 to 32. Results of ability for pyocyanin producing showed variance in the strength and the quantity at absorbance ranged from 0 to 0.93 at 400 nm. An extrusive Correlation is noticed among agglutinatinal activity for Lectins and protease, hemolysin, and pyocyanin pigment activities. So, the isolates which have high agglutionational activity are bioactive in protease, hemolysin and pyocyanin, and on the contrary | ||
| Keywords | ||
| pseudomonas aeruginosa; PCR; S rRNA; Sensitivity test; Lectins | ||
|
Statistics Article View: 41 PDF Download: 14 |
||