H. Abad, M., A. A. Al-haddad, Z. (2017). Isolation of Aspergillus flavus from maize and sesame with detection of aflatoxin B1 in rural area around Baghdad. , 10(2), 10-16.
M. H. Abad; Z. A. A. Al-haddad. "Isolation of Aspergillus flavus from maize and sesame with detection of aflatoxin B1 in rural area around Baghdad". , 10, 2, 2017, 10-16.
H. Abad, M., A. A. Al-haddad, Z. (2017). 'Isolation of Aspergillus flavus from maize and sesame with detection of aflatoxin B1 in rural area around Baghdad', , 10(2), pp. 10-16.
H. Abad, M., A. A. Al-haddad, Z. Isolation of Aspergillus flavus from maize and sesame with detection of aflatoxin B1 in rural area around Baghdad. , 2017; 10(2): 10-16.

Isolation of Aspergillus flavus from maize and sesame with detection of aflatoxin B1 in rural area around Baghdad

Al-Anbar Journal of Veterinary Sciences
Article 1, Volume 10, Issue 2, 2017, Pages 10-16    PDF (439.16 K)
Authors
M. H. Abad; Z. A. A. Al-haddad
Abstract
The results of this investigation have demonstrated the isolation and diagnosis of toxigenic Aspergillus flavus with detection of the aflatoxin B1 from maize and sesame samples in the rural area around Baghdad. A hundred sample of maize and sesame were collected from Abu Ghraib, Tagi, Tarmiya, Mahmoudiyah and Alabegi. Maize and sesames seeds were collected and mixed uniformly, After being mixed, 5seeds were randomly selected and surface sterilized in a 2.5% NaOCl solution for 1 min followed by washing two times in sterile distilled water, then dried by sterilized filter paper, Five complete grains were added via sterilized forceps into a previously prepared plate, containing sabouraud dextrose agar, Incubation of all plates for (7) days at 25 Cْ with intermittent observation of the fungal growth, plug of mycelium was aseptically removed from the orange medium below each infected seed and sub-cultured on Rose Bengal Agar (RBA) and incubation at 25°C for 5 days. Aspergillus flavus was isolated from maize sample in area around Baghdad, that obtained Aspergillus flavus in 5 samples in the total of 10 samples in Abu Ghraib, 3 samples from the total of 10 samples in Tagi, 6 samples of the total of 10 samples in Al Tarmia, 5 samples from the total of 10 samples in Mahmoudiya and 4 samples from the total of 10 samples in Alabegi while Aspergillus flavus was isolated from sesame sample in area around Baghdad, that obtained Aspergillus flavus in 2 samples in the total of 10 samples in Abu Ghraib, 3 samples from the total of 10 samples in Tagi, 1sample of the total of 10 samples in Al Tarmia, 4 samples from the total of 10 samples in Mahmoudiya and 2 samples from the total of 10 samples in Alabegi.detected of aflatoxin B1in maize sample in the area around Baghdad, whereby was obtained on 5 a contaminated samples with aflatoxin B1 from the total of 10 samples in Abu Ghraib, 3 contaminated samples with aflatoxin B1from the total of 10 samples in Tagi, 6 contaminated samples with aflatoxin B1from the total of 10 samples in Al Tarmia, 5 contaminated samples with aflatoxin B1from the total of 10 samples in Mahmoudiya and 6 contaminated samples with aflatoxin B1from the total of 10 samples in Alabegi while detected of aflatoxin B1in sesame sample in the area around Baghdad, whereby was obtained on 2 a contaminated samples with aflatoxin B1 from the total of 10 samples in Abu Ghraib, 2contaminated samples with aflatoxin B1from the total of 10 samples in Tagi, 1contaminated samples with aflatoxin B1from the total of 10 samples in Al Tarmia,4 contaminated samples with aflatoxin B1from the total of 10 samples in Mahmoudiya and 2 contaminated samples with aflatoxin B1from the total of 10 samples in Alabegi.
Keywords
Isolation; Aspergillus flavus; maize and sesame; detection of aflatoxin B
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