Effect of Ethanol Extract of Radish (Raphanus sativus L.) Seeds and Lactobacillus acidophilus on Enteropathogenic Escherichia coli in vitro and in vivo.

A. Taqi, Rami; K. Ghanima, Kais; N. AL.Thwani, Amina

	Effect of Ethanol Extract of Radish (Raphanus sativus L.) Seeds and Lactobacillus acidophilus on Enteropathogenic Escherichia coli in vitro and in vivo.
iraq journal of market research and consumer protection
Article 1, Volume 2, Issue 4, 2010, Pages 166-181
Authors
Rami A. Taqi; Kais K. Ghanima; Amina N. AL.Thwani
Abstract
The antibacterial activity of each of ethanol extract of radish seeds and L. acidophilus against Enteropathogenic E. coil was studied using well diffusion method (in vitro) and mice (in vivo), The growth kinetics of mixed culture of two bacterial species were also studied. The results showed a strong antibacterial activity against E. coli for each of ethanol extract of radish seeds (R. sativus) and L. acidophilus . It was found that the diameters of inhibition zones were 28 and 25mm. respectively .It was found also that the L. acidophilus inhibits the growth of E.coli in mixed culture, and the inhibition begun after 36 hr. but after 72 hr. there was no any growth of E. coil. The histopathological study of intestine and liver in mice revealed that the ethanol extract and L. acidophilus exhibited inhibition in the growth of E. coli and prevent any inflammation or harmful histological changes, Such findings confirms the benifical effects of Lactobacillus as probiotic and that ethanol extract of radish seeds as treatment compound for diarrheal diseases caused by bacterial pathogens. It was concluded that the extract and Lactobacillus holds great promise as antimicrobial and anti-inflammatory therapeutic

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