R. Abbas, M., M. K. Saadedin, S., A. Suleiman, A. (2024). MOLECULAR IDENTIFICATION OF ROOT-KNOT NEMATODES MELOIDOGYNE SPP. ISOLATED FROM CUCUMBER FARMS IN DIFFERENT IRAQI REGIONS. , 22(2), 1174-1191. doi: 10.32649/ajas.2024.149510.1245
M. R. Abbas; S. M. K. Saadedin; A. A. Suleiman. "MOLECULAR IDENTIFICATION OF ROOT-KNOT NEMATODES MELOIDOGYNE SPP. ISOLATED FROM CUCUMBER FARMS IN DIFFERENT IRAQI REGIONS". , 22, 2, 2024, 1174-1191. doi: 10.32649/ajas.2024.149510.1245
R. Abbas, M., M. K. Saadedin, S., A. Suleiman, A. (2024). 'MOLECULAR IDENTIFICATION OF ROOT-KNOT NEMATODES MELOIDOGYNE SPP. ISOLATED FROM CUCUMBER FARMS IN DIFFERENT IRAQI REGIONS', , 22(2), pp. 1174-1191. doi: 10.32649/ajas.2024.149510.1245
R. Abbas, M., M. K. Saadedin, S., A. Suleiman, A. MOLECULAR IDENTIFICATION OF ROOT-KNOT NEMATODES MELOIDOGYNE SPP. ISOLATED FROM CUCUMBER FARMS IN DIFFERENT IRAQI REGIONS. , 2024; 22(2): 1174-1191. doi: 10.32649/ajas.2024.149510.1245

MOLECULAR IDENTIFICATION OF ROOT-KNOT NEMATODES MELOIDOGYNE SPP. ISOLATED FROM CUCUMBER FARMS IN DIFFERENT IRAQI REGIONS

Anbar Journal of Agricultural Sciences
Volume 22, Issue 2, December 2024, Pages 1174-1191    PDF (997.07 K)
Document Type: Research Paper
DOI: 10.32649/ajas.2024.149510.1245
Authors
M. R. Abbas1; S. M. K. Saadedin2; A. A. Suleiman* 3
1Directorate of Seed Testing and Certification, Ministry of Agriculture, Baghdad
2Institute of Genetic Engineering and Biotechnology, University of Baghdad, Baghdad, Iraq
3College of Science, University of Anbar, Anbar, Iraq
Abstract
Meloidogyne spp. is one of the most common crop diseases globally. Due to the similarity in morphological features between various Meloidogyne spp., molecular techniques were used to identify their species. In this study, conducted in October 2022 and January 2023, genetic identification was undertaken on 61 samples collected from nine distinct regions of Iraq. PCR was used to amplify the part of the Meloidogyne spp. genome between the COI and 16S mtDNA genes. Afterward, M. incognita, M. hapla, M. javanica, M. enterolobii, and M. arenaria were genotyped using Sequence Characterized Amplified Region (SCAR-PCR), with decreasing prevalence rates, respectively. The data revealed that the 50 isolates of M. incognita were in the Shuan Kara region, which means they exhibited the highest percentage. Interestingly, M. arenaria was the least common species in all areas and was isolated from Ghanaia. Additionally, M. enterolobii's representation of other species led to the discovery of a new one in Iraq. The coexistence of more than two species within a single region has never been documented. The goal of this study is to use molecular detection to find out how different Meloidogyne species infect cucumber plants and how important it is to be able to spot infected regions.
Keywords
COI Barcode; Molecular Markers; mtDNA; Nematodes
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