J. Al-Attar, E., H. H. Alzobaay, A., K. Hasan, S. (2024). INVESTIGATION OF OPTIMAL CONDITIONS FOR THE PRODUCTION OF POLY GAMMA GLUTAMIC ACID (Γ -PGA) FROM A LOCAL ISOLATE OF THE BACILLUS MEGATERIUM. , 22(2), 1499-1513. doi: 10.32649/ajas.2024.184656
E. J. Al-Attar; A. H. H. Alzobaay; S. K. Hasan. "INVESTIGATION OF OPTIMAL CONDITIONS FOR THE PRODUCTION OF POLY GAMMA GLUTAMIC ACID (Γ -PGA) FROM A LOCAL ISOLATE OF THE BACILLUS MEGATERIUM". , 22, 2, 2024, 1499-1513. doi: 10.32649/ajas.2024.184656
J. Al-Attar, E., H. H. Alzobaay, A., K. Hasan, S. (2024). 'INVESTIGATION OF OPTIMAL CONDITIONS FOR THE PRODUCTION OF POLY GAMMA GLUTAMIC ACID (Γ -PGA) FROM A LOCAL ISOLATE OF THE BACILLUS MEGATERIUM', , 22(2), pp. 1499-1513. doi: 10.32649/ajas.2024.184656
J. Al-Attar, E., H. H. Alzobaay, A., K. Hasan, S. INVESTIGATION OF OPTIMAL CONDITIONS FOR THE PRODUCTION OF POLY GAMMA GLUTAMIC ACID (Γ -PGA) FROM A LOCAL ISOLATE OF THE BACILLUS MEGATERIUM. , 2024; 22(2): 1499-1513. doi: 10.32649/ajas.2024.184656

INVESTIGATION OF OPTIMAL CONDITIONS FOR THE PRODUCTION OF POLY GAMMA GLUTAMIC ACID (Γ -PGA) FROM A LOCAL ISOLATE OF THE BACILLUS MEGATERIUM

Anbar Journal of Agricultural Sciences
Volume 22, Issue 2, December 2024, Pages 1499-1513    PDF (964.57 K)
Document Type: Research Paper
DOI: 10.32649/ajas.2024.184656
Authors
E. J. Al-Attar; A. H. H. Alzobaay; S. K. Hasan
University of Baghdad, College of Agricultural Engineering Sciences, Department of Food Sciences
Abstract
This study involved the production of poly-γ-glutamic acid (γ-PGA) using a local isolate of the Bacillus megaterium bacteria. Two natural carbon sources were used for qualitative detection and quantitative estimation using thin layer chromatography [TLC]. The study also evaluated its ability to grow in a production medium and improve productivity by manipulating its components and environmental conditions using molasses (sugarcane), corn syrup, glucose, fructose, sucrose, and some sources of nitrogen. The best sources and optimal concentrations of carbon and nitrogen were selected. The optimal conditions for production of γ-PGA were by using a semi-synthetic medium by preparing the molasses as a carbohydrate source at 10% concentration, while ammonium nitrate provided the best source of nitrogen at 2%. This study showed that the optimum temperature and initial pH for achieving better γ-PGA productivity from the studied bacteria was at 37ᵒ C and 6.5, respectively. Higher productivity was achieved by agitation and aeration at a ratio of 5:1 of the fermentation media size to flask volume in the rotary shaker incubator at 180 rpm/min, using an inoculum size of 2.8×106 cfu/mL in the production medium till the total γ-PGA concentration reached 7.22 mg/ml. The γ-PGA was then extracted from a productive medium after precipitation and obtaining pure crystals, and its purity determined using Fourier-Transform Infrared Spectroscopy (FTIR)
Keywords
(γ-PGA); B. megaterium; Molasses; corn syrup; Fermentation; FTIR
References
  1. Abd Alsaheb, R. A., Othman, N. Z., Abd Malek, R., Leng, O. M., Aziz, R., and El Enshasy, H. A. (2016). Polyglutamic acid applications in pharmaceutical and biomedical industries. Der Pharmacia Letter, 8(9): 217-225.
  2. Abd Rahman, R. N. Z., Geok, L. P., Basri, M., and Salleh, A. B. (2005). Physical factors affecting the production of organic solvent-tolerant protease by Pseudomonas aeruginosa strain K. Bioresource technology, 96(4): 429-436. https://doi.org/10.1016/j.biortech.2004.06.012.
  3. Al-Attar, E. J., Salih, N. M., and Al-Rajhi, S. H. (2017). Food Applications of Monosodium Glutamate Salt Produced from Bacillus subtilis EN3A1-P19U7. Baghdad Science Journal, 14(1): 32. https://doi.org/10.21123/bsj.2017.14.1.0032.
  4. Al-Azzami, A. A., Yaseen, A. A., Gharbi, W. A., Mohammed, T. T., and Khaleel, N. T. (2024). The Effect Of Partial Replacement Of Mung Beans Jerusalem Artichoke Powder On Some Microbial And Sensory Properties Of Chilled Chicken Burgers. Tikrit Journal for Agricultural Sciences, 24(3): 333-342. https://doi.org/10.25130/tjas.24.3.26
  5. Ali, A., Usman, M., Jayamani, E., Soon, K., and bin Ab Wahab, M. K. (2024). Sustainable Biomanufacturing. In Biomedical Implants, 195-217.
  6. Alzobaay, A. H. H., Al-Attar, E. J., and Hasan, S. K. (2023). Effect of long storage of sinapis alba seeds aqueous extract in viability against spoiled bacteria in refrigerated ground beef. Anbar Journal of Agricultural Sciences, 12(2): 343-353. https://doi.org/10.32649/ajas.2024.141616.1056.
  7. Bang, B. H., Rhee, M. S., Kim, K. P., and Yi, D. H. (2012). Influences of culture medium components on the production poly (γ-glutamic acid) by Bacillus subtilis GS-2 isolated Chungkookjang. The Korean Journal of Food and Nutrition, 25(3): 677-684.
  8. Cai, M., Han, Y., Zheng, X., Xue, B., Zhang, X., Mahmut, Z., ... and Sun, J. (2023). Synthesis of poly-γ-glutamic acid and its application in biomedical materials. Materials, 17(1): 15. https://doi.org/10.3390/ma17010015.
  9. Chen, X., Chen, S., Sun, M., and Yu, Z. (2005). High yield of poly-γ-glutamic acid from Bacillus subtilis by solid-state fermentation using swine manure as the basis of a solid substrate. Bioresource technology, 96(17): 1872-1879. https://doi.org/10.1016/j.biortech.2005.01.033.
  10. Dike, K. S., Akim, D. I., and Ekwealor, I. A. (2013). Effect of vitamins and bivalent metals on growth and methionine productivity by Bacillus cereus. Journal of Microbiology and Biotechnology Research, 3(2): 88-95.
  11. Eggeling, L., Pfefferle, W., and Saham, H. (2001). Amino acids. In Basic Biotechnology. Ratledge, C., and Kristiansen, B. Cambridge Univ., Press. Cambridge, UK, 281-303.
  12. Elbanna, K., Alsulami, F. S., Neyaz, L. A., and Abulreesh, H. H. (2024). Poly (γ) glutamic acid: a unique microbial biopolymer with diverse commercial applicability. Frontiers in microbiology, 15: 1348411. https://doi.org/10.3389/fmicb.2024.1348411.
  13. Gutcho, S. J. (1973). Chemicals by Fermentation. Noyes Data Corp. New Jersey. P: 215.
  14. Jebur, H. A., and Auda, J. M. (2020). Evalution of antimicrobial activity of partial purified bacteriocin from local isolate of Bacillus licheniforims HJ2020 MT192715. 1. Iraqi Journal of Agricultural Sciences, 51(6).
  15. Li, D., Hou, L., Gao, Y., Tian, Z., Fan, B., Wang, F., and Li, S. (2022). Recent advances in microbial synthesis of poly-γ-glutamic acid: a review. Foods, 11(5): 739. https://doi.org/10.3390/foods11050739.
  16. Li, M., He, Y., and Ma, X. (2022). Separation and quantitative detection of fermentation γ-polyglutamic acid. Journal of Future Foods, 2(1): 34-40. https://doi.org/10.1016/j.jfutfo.2022.03.015.
  17. Mohammad, A., Moheman, A., and El-Desoky, G. (2012). Amino acid and vitamin determinations by TLC/HPTLC: review of the current state. Open Chemistry, 10(3): 731-750. https://doi.org/10.2478/s11532-012-0019-0.
  18. Moraes, L. P., Brito, P. N., and Alegre, R. M. (2013). The existing studies on biosynthesis of poly (γ-glutamic acid) by fermentation. Food and Public Health, 3(1): 28-36.
  19. Moreira, J. V., Silva, S. C. M., and Cremasco, M. A. (2020). Evaluation of carbon: nitrogen ratio in semi-defined culture medium to tacrolimus biosynthesis by Streptomyces tsukubaensis and the effect on bacterial growth. Biotechnology reports, 26: e00440. https://doi.org/10.1016/j.btre.2020.e00440.
  20. Mumtaz, M., Hussain, N., Ashraf, M., Azam, H. M. H., and Iftikhar, A. (2024). Introduction to Biopolymers, Their Blend, IPN s, Gel, Composites, and Nanocomposites. Applications of Biopolymers in Science, Biotechnology, and Engineering, 1-29. https://doi.org/10.1002/9781119783473.ch1.
  21. Nadeem, S., Niaz, B., Muzammil, H. M., Rana, S. S., Rajok, M. I., and Shakoori, A. R. (2011). Optimizing carbon and Nitrogen sources for L-Glutamic acid production by Brevibacterium strain NIABSS-67. Pakistan J. zool, 43(2): 285-290.
  22. Ogunleye, A., Bhat, A., Irorere, V. U., Hill, D., Williams, C., and Radecka, I. (2015). Poly-γ-glutamic acid: production, properties and applications. Microbiology, 161(1): 1-17. https://doi.org/10.1099/mic.0.081448-0.
  23. Qiao, C., Zhang, S., Li, Z., Chen, X., Li, X., and Lan, L. (2013). Improving poly-(γ-glutamic acid) production and reducing impurities in fermentation broth by medium optimization using Bacillus. licheniformis CGMCC3336. Journal of Biobased Materials and Bioenergy, 7(3): 390-394. https://doi.org/10.1166/jbmb.2013.1362.
  24. Rao, R., Sridevi, V., and Swamy, A. V. N. (2013). Statistical optimization of fermentation conditions for L-glutamic acid production by free cells of Corynebacterium glutamicum ATCC13032. International Journal of Engineering Science Invention, 2(9-Р): 23-28.‏
  25. Samargandi, D., Zhang XueJia, Z. X., Liu Feng, L. F., and Tian ShuGe, T. S. (2014). Fourier Transform Infrared (FT-IR) spectroscopy for discrimination of fenugreek seeds from different producing areas. Journal of Chemical and Pharmaceutical Research., 6(9): 19-24.
  26. Sarvamangala, D. D., and Vijayalakshmi, P. (2013). Production and crystallization of L-glutamic acid. Int J Pharm Sci Rev Res, 18: 58-60.
  27. Sundaramurthy, S. (2016). Mixing in Shake Flask Bioreactor. Encyclopedia of Industrial Biotechnology: Bioprocess, Bioseparation, and Cell Technology; Flickinger, MC, Ed, 1-17.
  28. Tork, S. E., Aly, M. M., Alakilli, S. Y., and Al-Seeni, M. N. (2015). Purification and characterization of gamma poly glutamic acid from newly Bacillus licheniformis NRC20. International journal of biological macromolecules, 74: 382-391. https://doi.org/10.1016/j.ijbiomac.2014.12.017.
  29. Waqas, M., Rehan, M., Khan, M. D., and Nizami, A. S. (2019). Conversion of food waste to fermentation products. Encyclopedia of food security and sustainability, 1: 501-509. https://doi.org/10.1016/B978-0-08-100596-5.22294-4.
  30. Younis, M. A., Hezayen, F. F., Nour-Eldein, M. A., and Shabeb, M. S. (2010). Optimization of cultivation medium and growth conditions for Bacillus subtilis KO strain isolated from sugar cane molasses. Am Eurasian J Agric Environ Sci, 7(1): 31-37.
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