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EFFET OF EXTENDERS AND CRYOGENES ON SOME SPERMATOLGICAL PROPERTIES OF GRASS CARP CTENOPHORYNGODEN IDLLA FISH | ||
| journal of kerbala university | ||
| Article 1, Volume 5, Issue 4, 2009, Pages 215-222 PDF (0 K) | ||
| Abstract | ||
| Experiment were carried out to investigate the effect of six extenders Modified kurokura (M.K) Artificial seminal plasma(ASP) Kurokura Immobilizing Media Glucose + Nacl + NaHco3 and Glucose + Tris And three cryoprotectant agents Dimethyl sulfa oxide (DMSO) ME2SO Methanol and Glycerol on the spermatological properties which are total motility % total viability (Duration of motility) /second Individual duration /second and pH of seminal plasma and packed sperm volume % and number of sperm x 109/ml3 of seminal plasma of pooled semen collected from proven selected brood stock males grass carp hormonally induce stripping at reproduction season (march , April and may) 2008 in Babylon province Hatcheries . The milt was diluted at 1; 10 total volume extender and 10 % cryogen equilibrate fore 10 minute then packed in plastic straw 0.25 ml sealed with poly venial alcohol and Cryopreservation at – 196 c°. This study showed a significant influence of extender and cryogen on the spermatological properties of preserved semen , the optimum post thawing motility % after activation which is one of key that influence on fertilizing ability of sperm when used the modified kurokura and artificial seminal plasma (55.55 ,53.88 ,50.00 ,45.55 and 40.00) (56.11 ,45.44 ,50.00 ,45.55 and 38.88 %) respectively from June to October and the optimum result when used DMSO with extender contain salt and ions (55.00 ,53.61 ,50.55 ,47.77 and 42.22%) respectively from June to October DMSO It is conclude from this study that long term cryopreservation at -196 °c can be successfully done . The present in formation will evently help in selecting good extender M. K and A.S.P and with DMSO as crygene for long term cryo presertion for improved artificial propagation in grass carp fishs . | ||
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