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Antibacterial and Molecular Docking Analysis of Biosurfactants Produced from Lactobacillus Acidophilus Against MDR Staphylococcus aureus | ||
| Al-Qadisiyah Journal of Veterinary Medicine Sciences | ||
| Volume 24, Issue 2, December 2025, Pages 58-71 PDF (735.63 K) | ||
| Document Type: Research Paper | ||
| DOI: 10.29079/qjvms.2025.162826.1100 | ||
| Authors | ||
| Sumod Abdul Kadhem Salman* 1; Balsam Miri Mizher Al-Muhana* 2 | ||
| 1Deptartment of Microbiology, College of Veterinary Medicine, University of Al-Qadisiyah, Al-Diwaniyah, Iraq | ||
| 2University of AL-Qadisiyah College of Veterinary Medicine, Microbiology | ||
| Abstract | ||
| Lactic acid bacteria are members of probiotic bacteria and their metabolites as biosurfactants (BSS) emerged as great antibiotics alternatives. The current study aimed to produce, purify and evaluate the antibiofilm effect of BSs produced by Lactobacillus acidophilus isolate against multidrug resistant (MDR) Staphylococcus aureus. BSs were screened by preliminary tests like emulsification assays, oil spreading test and foaming activity. Purification of BSS was done by silica column chromatography, analyzed by FTIR and Gas chromatography–mass spectrometry. Broth macro-dilution method was employed to find Minimum inhibitory concentration (MIC) of BSS. Quantitative Reverse Transcriptase Real-Time PCR was to study expression for fib gene implicated in biofilm formation of 30 isolates of MDR S. aureus before and after treating these isolates with three concentrations of sub-MIC of BSS. Emulsification assay for BSS was 67%, clear zone (diameter 7 cm) was formed on the surface of petri dish contain oil-water interface. The foaming activity displayed percentage of 60%. FTIR analysis proposed that it composed of glycolipoproteins. GC-MASS showed presence of different biologically compounds as Benzoic acid, n-Hexadecanoic acid, Linoelaidic acid. The MIC value of BSS against MDR S.aureus isolates was 0.625 mg/mL. The results revealed that 26 (86.6%) of MDR isolates were positive for biofilm production. Only 4 (13.3%) of isolates produced biofilm after treating with sub-MIC of BSS. Chemical compounds in BSS was exposed to the molecular docking analysis against one of biofilm protein (fib protein) of S.aureus. Benzoic_acid possess good affinity toward fib protein (binding energy is 7.5). | ||
| Keywords | ||
| Probiotic; Biosurfactants; MDR; S. aureus; biofilm | ||
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