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Sequencing strain Fasciola species in Cattle in Iraq | ||
| Basrah Journal of Veterinary Research | ||
| Volume 25, Issue 1, March 2026, Pages 88-100 PDF (1.1 M) | ||
| Document Type: Research Paper | ||
| DOI: 10.23975/bjvr.2026.165734.1250 | ||
| Author | ||
| Ismael WadUllah Alsae'ag* | ||
| Middle Technical University\ College of Health and Medical Techniqes\ Baghdad | ||
| Abstract | ||
| Fascioliasis is a significant zoonotic disease caused by Fasciola species that affects the human and animals like sheep, cows and buffaloes worldwide that causes public and veterinary health problems. The aim of this study focusing on the genetic differentiation between the two species of Fasciola (F. hepatica and F. gigantica) by using large subunit of 28S rDNA gene. A total of 45 adult worms were isolated from liver of different cattle in Iraq-Basrah Provence, extraction of genomic DNA using special kit was performed to isolated Fasciola species with 21 bp specific primers included that target 28S rDNA. The BLAST and PCR products showed evolutionary phylogenetic and variation genetically with similarity in about 80% between the two species of Fasciola. The results phylogenetically concluded a highly species specific conservation and polymorphism with possible strain hybridization | ||
| Keywords | ||
| F. hepatica; F. gigantica; BLAST; rDNA; ITS | ||
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