Study of theImmunopathological Changes Induced by Pseudomonas aerugenosa in Mice Treated with Alcoholic Apium greveolense extract and Immunized with Whole Sonicated Salmonella typhimurium Antigens

H. Al- Kutbi, S.; S. Al- Najjar, S.; A. Al- Khafaji, M.; J. Alwan, M.

	Study of theImmunopathological Changes Induced by Pseudomonas aerugenosa in Mice Treated with Alcoholic Apium greveolense extract and Immunized with Whole Sonicated Salmonella typhimurium Antigens
Al-Anbar Journal of Veterinary Sciences
Article 1, Volume 3, Issue 1, 2010, Pages 43-55 PDF (310.43 K)
Authors
S. H. Al- Kutbi; S. S. Al- Najjar; M. A. Al- Khafaji; M. J. Alwan
Abstract
In order to know the efficacy of whole sonicated antigen with or without celery extract on the challenge with viable virulent Pseudomonas aeruginosa , thirty-five healthy white mice, both sex, aged between 6-8 weeks were randomly divided into five groups and treated as following: The 1st group (n= 10) immunized with WSSAgs (0.5ml/lmg protein) S/C as primary dose followed by second dose after two weeks. 2-The 2nd group(n=10) administrated daily dose of 0.5ml of alcoholic celery extract (Concentration of protein 600 mg/kg BW) orally for 30 days and at the same time treated as 1st group. 3- The 3rd group (n=5) administrated orally with alcoholic celery extract only with same dose and time of the 2nd group. The 4th and 5th group (5 animals each) treated as control positive and negative respectively. Cell mediated and humoral responses were checked at day 27 and day 30 post-immunization, at day 3o post immunization, the 1st, 2nd, 3rd and 4th group were challenged with 0.5 ml of bacterial suspension content 1X10 CFU of viable virulent Pseudomonas aeruginosa S/C and the 5th group inoculated S/C with 0.5 ml of sterile normal saline and served as control negative group. All animals were sacrificed at day 20 post-challenge ,post-mortem examined and bacterial and pathological examination of their internal organs were done. The results explained that WSSAgs induced cellular and humoral immune response in immunized animal and these values was high in animal pretreated with alcoholic celery extract and immunized with WSSAgs. All non immunized and pretreated alone infected animals died during 24-48hrs post-inoculation with severe bacterial isolation from internal organs as compared with immunized and pretreated immunized infected animals which showed good healthy during the course of experiment and no bacterial isolation from internal organs. Multiple granulomatous lesion were seen in the internal organs of immunized infected animals while the pretreated immunized infected animals showed lymphocytic aggregation around blood vessels and in the parenchyma of most examined organs ,in contrast, the non-immunized and pretreated infected animals showed acute suppurative reaction with thrombus formation in most examined organs.

Statistics Article View: 151 PDF Download: 3