The effect of life and dead cell and cell free extract for same lactic acid bacteria in reducing cholesterol and microbial load for buffalo fatty tissue

A. M. Al-Sheikh Thahir, A.

	The effect of life and dead cell and cell free extract for same lactic acid bacteria in reducing cholesterol and microbial load for buffalo fatty tissue
Al-Anbar Journal of Veterinary Sciences
Article 1, Volume 4, Issue 1, 2011, Pages 109-116 PDF (402.37 K)
Author
A. A. M. Al-Sheikh Thahir
Abstract
Some of chemical composition for abdominal buffalo fatty tissue were measured (moisture, fat, protein, and ash) the results were 9.7, 84.79, 3.02 and 1.33% respectively. While the cholesterol level, peroxide value, pH were 178.62 mg/100gm fat, 2.73 ml meq O2/kg fat and 6.27 respectively. 10% inoculums of life and dead bacteria (which killed by using slow pasteurization 63oC for 30 minute) and cell free extract (CFE), four lactic acid bacteria (Lactobacillus casei, Lactobacillus delbrueckii spp bulgaricus, Lactobacillus acidophilus and Bifidus actiregularis), added to abdominal buffalo fatty tissue and incubated at 37oC for 48 hours, the results were shown decreasing in cholesterol level between 28.16 – 60.34% for the life bacteria, Lb.caseiwas the most efficient, for the CFE treatments the percentage decreasing were between 17.28- 26.45% Lb.acidophiluswas the best, while the decreasing in cholesterol level for the dead bacteria treatments were between 9.11– 21.32 %, Lb. bulgaricus was the most effective. The same treatments also shown high efficiency in reducing total coliform, lipolytic bacteria and yeasts and molds as the microbial load indicators in the abdominal buffalo fatty tissue, Lb.acidophilus and Lb. bulgaricus were the best among the other bacteria.

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