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PRODUCTION OF HYDROCELLULASE FROM LOCAL ISOLATE OF ASPERGILLUS SP | ||
| The Iraqi Journal of Agricultural Science | ||
| Article 1, Volume 38, Issue 4, 2007, Pages 105-110 | ||
| Author | ||
| Aswan H. Aboud Albayar | ||
| Abstract | ||
| A hydrocellulase enzyme was produced from local isolate of Aspergillus sp., by solid state fermentation by using maize cobs as ap suport material and wheat straw treated with alkaline peroxide, pH=6.8,vith inoculum size of 106 spore/gm at 30°C for 7 days. After enzyme extraction with phosphate buffer it was purified among cellulases first by ammonium sulphate 40% saturation, next by gel filtration by Sephadex G-200 (60xl.5cm), then it was purified by ion exchanger DEAE-Sephadex A50 (8x2.2cm). Enzymatic activity of purified enzyme was estimated by using CMC and insoluble cellooligosaccharides as substrates. It was convinced as hydrocellulase by using insoluble cellooligosaccharides as a substrate, its activity was 17.54 U/ml comparatively with using CMC which was 11.76 U/ml. The specific activity of purified enzyme was 115.95 U/mg. In conclusion, this type of enzymes are active on non-crystalline cellulosic materials, so the produced cellubiose b y cellulases will analyzed to glucose so it will decrease the inhibition effect of cellubiose on other cellulases. This kind of cellulases released glucose out of the non reducing ends of oligosaccharides. Endoglucanases are responsible of decreasing the degree of cellulose polymerization because they are broke cellulosic chains from inside of non-crystalline regions. The existence of two kinds of exoglucanases is necessary to complemize the effect of each other, one of is them active on reducing ends while the other is active on non reducing ends. As a result of the this action we can use this kind of cellulases in sugar production because of its important roles in glucose production from cellulose, beside ethanol production biological fuel, some drugs and pharmatical materials, paper industries and fibers. | ||
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