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Detection of Bovine Viral Diarrhea Virus by Conventional RT-PCR: A comparative Study | ||
| Al-Anbar Journal of Veterinary Sciences | ||
| Article 1, Volume 4, Issue 2, 2011, Pages 121-128 PDF (165.99 K) | ||
| Author | ||
| K. S. A. Al-Ajeeli | ||
| Abstract | ||
| Fifty serum and buffy coat samples were collected from bovine from illegal abattoirs around Baghdad. RNA extraction was performed on buffy coat samples. These samples were subjected to conventional RT-PCR for the detection of BVDV. The result showed that the BVDV was detected by RT-PCR in three samples only. A comparison study was also conducted on the sensitivity of RNA extracted and non-extracted buffy coat and serum samples in conventional RT-PCR. The result showed that RNA extraction is the method of choice when conventional RT-PCR was performed on blood samples from BVDV infected animals. In a final conclusion, BVDV was detected by RT-PCR in local bovine. The findings suggested and proposed epidemiological studies on BVDV in Iraq in conjunction with isolation of the virus and identification of its genotype and biotype | ||
| Keywords | ||
| BVDV; PCR; bovine viral diarrhea | ||
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