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Isolation and diagnosis of Sinorhizobium meliloti symbiont of alfalfa with the study of effect salinity and pH on it | ||
| Journal of Basrah Researches (Sciences) | ||
| Article 1, Volume 36, Issue 3, 2010, Pages 1-13 PDF (0 K) | ||
| Authors | ||
| Jabbar Dehri; Ghosoon Fadhil; Basil Abd-Alzahraa Abbas | ||
| Abstract | ||
| Sixty local isolates of Sinorhizobium melilote.were isolated from Medicago sativa root nodules.The colonies characteristics were studied after growing on trypton yeast extract agar (Ty).All isolates were gram negative, rod shape and motile because of formation of cyclic B-(1-2)glucans.All isolates showed ability for production of cellulose fibrils and nonproduction of cyclic B-(1-3)glucans.The bacterial isolates formed nodules on infected plants after two weeks .Adding of differ at antibiotics to the cultural media in different concentration lead to inhibit the growth of some of the bacteria isolates.All isolates showed agood growth on media having 2% and 3% NaCl, but the growth was inhibited at 7% NaCl.regarding K2SO4 all isolates shawed growth on media containing 2%,3% and 4%K2SO4 but no growth was observed on 7.5%K2SO4 .pH 4 inhibit the growth of all isolates in contrast there was agood growth at pH ranging for 6 to 10.The total nitrogen of plant inoculated with bacterial isolates were determined of the soil characteristics were recorded. | ||
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