Histopathological  effects of  Citrobacter  freundii  in mice

	Histopathological effects of Citrobacter freundii in mice
journal of kerbala university
Article 1, Volume 6, Issue 2, 2010, Pages 103-119 PDF (0 K)
Abstract
The research carry out the investigation of histopathological effects of Citrobacter freundii in mice ,the isolate used was obtained from neonate suffering from acute watery diarrhea whom general stool examination showed no parasites,no fungi but culture showed heavy growth of Citrobacter freundii . The isolate was identified according to morphological and biochemical properties and the identification was confermed by using Api 20 E system. In order to know the sublethal dose for mice intraperitoneally injection, the LD50 experiment was done for Citrobacter freundii which was about 3.16×106 cell/mouse. Mice were injected with 106 cell/mouse and grossly and histopathological changes of selected organs were studied. The microscopic examination of stained tissue sections of organs obtained from mice injected with sublethal doses of Citrobacter freundii revealed that this bacteria have severe effects in the tissues obtained from (liver, lung, intestine and kidney). In liver there was odema, hemorrhage, immune cells migration and necrosiswhich is seen also in kidney. The main histopathological changes that seen in spleen was the agrigation of megakaryocytes but in lung there was acute bronchiolitis, cells within the lung tissue and increase of macrophages numbers. It was clear from these results that Citrobacter freundii caused systemic infection which indicated by the reisolation of this organism from mice organs in different ratios.

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