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Study the effects of some growth factors on the production of Xanthan by the bacterium Xanthomonas campestris using alkali hydolysate of date extract | ||
| journal of kerbala university | ||
| Article 1, Volume 1, Issue 0, 2005, Pages 69-77 PDF (0 K) | ||
| Abstract | ||
| This study was designed to produce microbial polysaccharide xanthan by using Xanthomonas campestris, which is used widely in food and pharmaceutical industries as well as in oil extraction using alkali hyrdolysate of date extract as a carbon source for the growth of bacteria and production of the polysaccharide. In addition, the objective of this study was to enhance the production of xanthan by the bacteria by finding the most favorable conditions of agriculture. It was revealed that the most optimal incubational period was the fifth day where 3.7 g/l was produced (75.57%). Upon addition of different concentrations of extract of fermentation to the medium, the production was increased up to 4.71 g/l (80.47%). It was further increased on addition of 0.4% of magnesium phosphate 0.4% to the nutritional medium. It was shown that ammonium phosphate was the best tested nitrogen source, where 11.19 g/l of xanthan was produced. It was also found that the revolution speed of centrifuge has a stimulating effect on the production of xanthan, in which 200 revolutions/min increased the production up to 13.03 g/l (154%). | ||
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