| This study was conducted to show the inhibitory effects of the aqueous extract of the grape seeds of three concentration (50, 100, 200 mg/ml) on the viable numbers of E.coli, Salmonella. paratyphi, and Staphylococcus aureus. These concentration of the extract mixed with the bacterial species for three periods of time (20, 40,60 min ) to notice the exposure time effect of the extract on the bacterial species inhibition. The results showed that there is a significant inhibition for grape seed extracts on the three species of bacteria. Also, the result showed that there is also significant inhibitory effects for the increase of the concentration of the extract . Also, the inhibitory effect increase with exposure time increase especially with Salmonella. paratyph and Staphylococcus aureus, however, E.coli showed less sensitivity concentration the time but E.coli more sensitivity concerning the time,but,E.coli more sensitive concerning the concentration of the extract. Moreover, the result showed that the extract have no Sabonification and Coumarin, but it is contain Tannins, Resin, Flavonoids, phenol and Glycoside compounds . |