Bioassay of the Beauveria bassiana(Bals.)Vuill fungi on the Fig moth Ephestia Cautella (Walk. ((Lepidoptera: Pyralidae) in vitro

	Bioassay of the Beauveria bassiana(Bals.)Vuill fungi on the Fig moth Ephestia Cautella (Walk. ((Lepidoptera: Pyralidae) in vitro
journal of kerbala university
Article 1, Volume 9, Issue 4, 2013, Pages 190-196 PDF (0 K)
Abstract
A bioassay for three different concentrations (1X102, 1X104, 1X106) spore/ml. of the water suspension of the Beauveria bassiana (Bals.)Vuill fungi spores on the eggs, 1st. and last instars, pupas and the adults of fig moth Ephestia Cautella (Walk.) in vitro. Monitoring the treated eggs and the hatching larvae lasted 5 days and showed that he least hatching percentage was 29.8% at 1X106 spore/ml. and the mortality of larvae peaked to 21.5% with the concentration 1X106 spore/ml. after 3 days. The rest of larvae were monitored till pupation and adult. Mortality of the 1st. instar peaked at 73.14% after 7 days of the 1X106) spore/ml. treatment but it's minimum was 2.4% one day after the 1X102 spore/ml. treatment application. 1X106 spore/ml. Treatment showed the highest effect on the 4th. Instar after 3 days of treatment so mortality was 76.5% , least pupation percentage 69.8% and the least emerge of adults was 64.2%. For pupa treatments, also the concentration 1X106 spore/ml. treatment showed the least emerge of adults 7 days after treatment with 16.22% and the highest effect in the adults mortality was 68.22% 7 days later too. Maximum mortality for adults was 68.22 seven days post treatment with 1X106 spore/ml.. Minimum adult mortality was 4.33 for the treatment with 1X104 spore/ml. one day after treatment.

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