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INHIBITORY EFFECT OF THE DOUBLE CONCENTRATED FILTRATE OF LBACTOBACILLUS CASEI IN VIVO AGAINST SALMONELLA TYPHIMURIUM | ||
| The Iraqi Journal of Agricultural Science | ||
| Article 1, Volume 45, Issue 4, 2014, Pages 384-392 | ||
| Authors | ||
| E. F. Hussein; H. A. Abdul-Ratha | ||
| Abstract | ||
| This study was carried out to investigate the therapeutic role of the double concentrated filtrate of Lb. casei toward Salmonella typhimurium in vivo .To achieve this goal this study included two main aspects as follow: 1. First aspect isolation and identification of Salmonella typhimurium from diarrhea infection of calves. For this purpose, 15 samples of faeces from infected calves were collected from Al-Fayhaa cattle station at Sowara and animal field of Agriculture College, University of Baghdad. These faecal samples were inoculated onto selected media including solid salmonella-shigella medium,xylose lysine deoxycholate XLD medium and solid MacConkey medium then incubated at 37˚C for 24 hr. According to the growth features and the microscopical examination of gram stained cells, and some biochemical and serological tests, only one isolate of Salmonella typhimurium from the diarrhea cases of calves was isolated. 2. The second aspect demonstrate inhibitory effect of the double concentrated filtrate of Lb. casei in vivo toward Salmonella typhimurium. Mice were infected and the histopathological changes of intestine, liver and spleen were recorded. The most important histopathological changes in these organs were congestion, bleeding and infiltration of inflammatory cells in addition to presence of coagulative necrosis in the liver and depletion of the white pulp in the spleen. Whereas the group of mice treated with double concentrated filtrate of Lb. casei after infected with Salmonella typhimurium showed improvement in the tissue of intestine, liver and spleen. | ||
| Keywords | ||
| Inhibitory effect; concentrated filtrate; Salmonella Typhimurium | ||
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