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ISOLATION AND IDENTIFICATION OF LOCALLY ISOLATE OF Erwinia cartrovora spp. AH88 FOR ASPARAGINASE PRODUCTION | ||
| IRAQ JOURNAL OF AGRICULTURE | ||
| Article 1, Volume 19, Issue 7, 2014, Pages 0-0 | ||
| Authors | ||
| A. K. faisal; H. A. Jebur | ||
| Abstract | ||
| Fifteen of locally isolates among 100 isolates were obtained from different sources of potato fields and storages in College of Agriculture and potato refrigerated storage in Abu –Grab markets. These isolates were distinguished as a clear colony, Shine leather surface, white -yellow color, rod and gram negative. These isolates were subjected to primary and secondary screening to obtain more efficient isolates that have high ability for producing asparagines. It was found that the isolate A88 had the highest enzyme activity and specific activity being 52.7 unit/ml and 8.0 U/ mg respectively, in comparison with isolate A73 which had the lowest of enzyme activity 33.4 U/ml and the specific activity was 1.1 U/mg. A morphological and Biochemical tests were performed for isolate A 88 to identify and evaluate it's origin.The results indicated that this isolate belong to Erwinia carotovora, and designated as Erwinia carotovora ssp. AH88. | ||
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