NUTRITIONAL AND CHEMICAL CONTENT OF TARAMIRA (Eruca sativa L.)  LEAVES EXTRACT AND ITS EFFECTIVENESS AGAINST SOME BACTERIAL SPECIES

M. Reshan, A.; A. Aziz, R.; S. Al-Temimi, S.

	NUTRITIONAL AND CHEMICAL CONTENT OF TARAMIRA (Eruca sativa L.) LEAVES EXTRACT AND ITS EFFECTIVENESS AGAINST SOME BACTERIAL SPECIES
IRAQ JOURNAL OF AGRICULTURE
Article 1, Volume 19, Issue 7, 2014, Pages 0-0
Authors
A. M. Reshan; R. A. Aziz; S. S. Al-Temimi
Abstract
This study has been performed to study the inhibitory effects of crude plant extracts of Taramira (Eruca sativa) leaves against some bacterial isolates represented by Staphylococcus aureus, Staphylococcus epidermids, Bacillus subtilis, Proteus vulgaris, Escherichia coli and Pseudomonas aeruginosa in vitro. The results showed that percentages of essential chemical of Eruca sativa leaves which represented by moisture, total oil, total ash, crude protein, crude fibers, carbohydrates and caloric values in dry weight are: 6.68, 12.69, 16.6, 28.87, 13.8, 35.16 % and 370.33 kcal/100g, respectively, the percentages of some major and minor mineral elements of Eruca sativa leaves powder which represented by Mn, Fe, Cu, Pb, Cd and As, are: 0.223, 0.232, 0.035, 0.008, 0.0007 mg/g and 3.465 ppm, respectively, while Co did not appear in the Eruca sativa leaves. The aqueous extract of the plant was acidic, its pH 5.53. The results of initial detection (precipitation) showed that ethyl acetate and aqueous extracts of Eruca sativa leaves contain all active compounds represented by tannins, saponins, flavonoides, glycosides, phenols, terpenoids and sterols, while alcoholic and hexane extracts contain also the some compounds except saponins, hexane extract contains no phenols. Through the study of the effect of Eruca sativa leaves extracts against growth of bacteria in vitro a significant difference at the degree of freedom 0.05 was observed, It was found that ethyl acetate and alcoholic extracts more were effective on the bacterial species than the other remaining extracts against the growth of bacteria, inhibitory activity was of all bacterial species at a concentration of 3.125 mg/ ml and it was more effective on Escherichia coli for ethyl acetate and alcoholic extracts giving inhibitory zone estimated 14.5,15.5 mm respectively, while hexane and aqueous extracts were contrasted their influence against all bacterial species had been impacted Escherichia coli and Pseudomonas aeruginosa only at a concentration of 3.125 mg/ml and diameter 11 mm from the extract of hexane, while appeared the effect of the bacterium Escherichia coli, Pseudomonas aeruginosa were effected only at a concentration of 50 mg/ ml and diameter 15, 13 mm, respectively, Bacillus subtilis was not affected in all concentrations of aqueous extract. When measuring the minimum inhibitory concentration (MIC) for each bacterial species at a concentrations of 6.25- 50 mg/ml for ethyl acetate and alcoholic extracts have high inhibitory activity compared with the other concentrations, but the hexane and aqueous extracts have affected bacterial species at 50- 100 mg/ ml, except for Staphylococcus aureus and Bacillus subtilis, which was not affected by any of the concentrations of extracts.

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