Alcoholic extraction of olive leaf and determination of antioxidant and antimicrobial activities

N. A. Al-Saidi, R.; A. L. Alasadi, H.

	Alcoholic extraction of olive leaf and determination of antioxidant and antimicrobial activities
Al-Anbar Journal of Veterinary Sciences
Article 1, Volume 8, Issue 1, 2015, Pages 64-70 PDF (664.64 K)
Authors
R. N. A. Al-Saidi; H. A. L. Alasadi
Abstract
The aim of this study was to extract olive leaf Where the extraction percentage was 7.24% in the use of solution 80% ethanol, 6% in 20% ethanol. Analyses of antioxidant activity was determine The quantity of the whole phenols in the extraction and it was 34 (mg/kg) as Gallic acid in the use of extraction solution 80% ethanol. The peroxide value (PV) for the extraction solution 20%, 80% ethanol was 5.6, 5.8, 4.2, 5.5 respectively when used in the concentration 50 and 100 (ppm). The result of High pressure liquid chromatography (HPLC) analyses, the phenolic compound for olive leaf extract 80% ethanol were Oleuropein, Apigenin-7-O-glucoside, Verbascoside, Luteolin-7-glucoside, in this study Quantities 60.2, 16.5, 10.9, 9.8 (mg/g.d.wol), respectively. The determinetion of antimicrobial activity to olive leaf extract against gram positive bacteria (staphylococcus aureus.) and gram negative bacteria (Pseudomonas sp. and E-coli) and on Yeast Candida-albicans it shown no activity for the 20% ethanol extract while 80% ethanol extract shown antimicrobial activity compare to Gentamycin antibiotic in the same concentration
Keywords
Olive leaf extract; Antioxidant activity of olive leaf extract; Antimicrobial activity of olive leaf extract

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